Alkyne-ethyl-PEG1-t-Butyl ester has an alkyne group and a t-butyl protected carboxyl group. The alkyne reacts with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Thecarboxyl group group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Alkyne-ethyl-PEG1-t-Butyl ester has an alkyne group and a t-butyl protected carboxyl group. The alkyne reacts with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Thecarboxyl group group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Low Abundance DNA Quantification Kit offers a PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range. The kit consists of a specially designed primer mix, that is used in conjunction with the provided 2x PCR Master Mix, to amplify human DNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown DNA is accurately quantified by using a standard curve constructed from the provided DNA Standard.
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Storage Conditions
Upon receipt, store Norgen’s Low Abundance DNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycle. If needed, prepare smaller working aliquots and store at -20°C or lower.
| Component | Cat. 57200 (48 reactions) |
|---|---|
| 2X PCR Master Mix | 1 mL |
| DNA Quantification Primer Set Mix | 200 µL |
| Quantified DNA Standard | 5 standards, each 100 µL |
| Nuclease-Free Water | 1.25 mL |
| Product Insert | 1 |
DBCO-PEG4-TFP ester serves as a heterobifunctional PEG linker containing an azide-reactive DBCO click chemistry handle and an amine-reactive TFP ester that is less susceptible to undergo hydrolysis compared to NHS ester. The PEG spacer enhances the water-solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-TFP ester serves as a heterobifunctional PEG linker containing an azide-reactive DBCO click chemistry handle and an amine-reactive TFP ester that is less susceptible to undergo hydrolysis compared to NHS ester. The PEG spacer enhances the water-solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture.
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 1mg plasmid DNA from 200ml bacterial culture |
| Applications | Enzyme digestion, sequencing, PCR and labeling, etc. |
| Purification method | Maxi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Conventional plasmid, plasmid less than 30KB |
| Sample amount | 100-200ml |
| Yield | 0.4-1mg |
| Elution volume | ≥500μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 20ml |
| Binding yield of column | 1mg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
| Contents | P101402 | P101403 |
| Purification Times | 10 Preps | 50 Preps |
| RNase A | 20 mg | 40 mg |
| Buffer P1 | 140 ml | 2 x 350 ml |
| Buffer P2 | 140 ml | 2 x 350 ml |
| Buffer LEN3 | 70 ml | 350 ml |
| Buffer GBT | 120 ml | 550 ml |
| Buffer PW1 | 60 ml | 300 ml |
| Buffer PW2* | 50 ml | 4 x 100 ml |
| Elution Buffer | 20 ml | 120 ml |
| HiPure DNA Maxi Columns III | 10 | 50 |
| Lysate Clear Maxi Syringe | 10 | 50 |
| 50 ml Collection Tubes | 20 | 100 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture.
