Propargyl-PEG8-amine

The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).

The kit uses linear dsDNA libraries (MGI platform) as input and enriches the circularized single-stranded DNA libraries. The circularization kit has a higher library circularization efficiency (25%) as compared to other vendors (7-15%).

NGS Library Circularization Kit workflow

Comparison of Library Circularization Efficiency

Kit features

• • • Fast protocol
      • Total protocol time is around 1 hour
      • Hands-on time is only ~10 minutes
    • Guaranteed high library circularization efficiency as compared to other kits
    • Input DNA amount: 100-300 ng

The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).

Propargyl-PEG13-acid consists of an alkyne group and a carboxylic acid. The carboxylic acid reacts with amine groups to form amide bonds in the presence of EDC or HATU as an activator. The alkyne group is commonly used in copper catalyzed azide-alkyne Click Chemistry reactions. The hydrophilic PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG13-acid consists of an alkyne group and a carboxylic acid. The carboxylic acid reacts with amine groups to form amide bonds in the presence of EDC or HATU as an activator. The alkyne group is commonly used in copper catalyzed azide-alkyne Click Chemistry reactions. The hydrophilic PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.

Standard LyoBeads contain DNA polymerase(s), reaction buffer and dNTPs. LyoBeads can also contain Primer & Probes when produced customized. They are rehydrated within seconds in any aqueous solutions, which  makes reaction setup very simple. Only a biological sample has to be added.

Storage: LyoBeads are shipped and stored simply at room-temperature. This provides a more cost efficient and ecological distribution compared to other master mixes.

LyoBeads can be pre-dispensed in PCR-strips, PCR-plates, cartridges etc.

All necessary components for PCR are already included in one LyoBead: an engineered DNA polymerase, an optimized reaction buffer and ultrapure dNTPs. Only primers and probes need to be added. A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction set-up.

For research use and further manufacturing.

In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.

LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.

Standard LyoBeads contain DNA polymerase(s), reaction buffer and dNTPs. LyoBeads can also contain Primer & Probes when produced customized. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very simple. Only a biological sample has to be added.