Propargyl-PEG12-amine is a heterobifunctional reagent that enables amide formation with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group is reactive with azide compounds via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The PEG units enhance the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG12-amine is a heterobifunctional reagent that enables amide formation with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group is reactive with azide compounds via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The PEG units enhance the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Intended Use
For the isolation of Listeria spp. from food and environmental specimens.
Principle and Interpretation
Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; aesculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.
Formulation
| Ingredients | /liter |
| Enzymatic Digest of Animal Tissues | 5.0g |
| Enzymatic Digest of Casein | 5.0g |
| Meat extract | 5.0g |
| Yeast extract | 5.0g |
| Sodium chloride | 20.0g |
| Disodium hydrogen phosphate dihydrate | 12.0g |
| Potassium dihydrogen phosphate | 1.35g |
| Aesculin | 1.0g |
| Lithium chloride | 3.0g |
| pH7.2±0.2 at 25°C | |
Preparation
Dissolve 57.4g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes. Then cool to 50°C，and add one Supplement(SR0120) per 225mL of base to prepare Half-Fraser Broth.
Quality Control
Cultural characteristics observed after incubation at 29-31°C for 22~26 hours
| Quality control strains | Growth | Characteristics |
| Listeria monocytogenes ATCC19115 | >20 cfu On PALCAM | Gray to black colony count with black halo |
| Escherichia coli ATCC25922 | < 100 colonies on TSA | Inhibition |
| Enterococcus faecalis ATCC29212 |
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the isolation of Listeria spp. from food and environmental specimens. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nit……
10 Layer Cell Factory One Wide Mouth And One Narrow Mouth
Huayi Cell Factory is a robust and easy-to-use cell
culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
PRODUCT FEATURES
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
Huayi Cell Factory is a robust and easy-to-use cell
culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
