Propargyl-PEG12-amine is a heterobifunctional reagent that enables amide formation with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group is reactive with azide compounds via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The PEG units enhance the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG12-amine is a heterobifunctional reagent that enables amide formation with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group is reactive with azide compounds via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The PEG units enhance the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The DirectBlood Genotyping Kit is compatible with a wide range of assays: Here we are detecting a SNP in the Factor V gene (rs6025; G1691A), which is connected with a higher risk for venous thrombosis, with a fluorescent hydrolysis probe assay.
Homozygous wild type blood samples will show a strong signal and amplification plot (blue curve) in channel 2 (Cy5) and none or a very low signal
(red curve) in the other channel 1 (ROX).
Heterozygous blood samples will give an intermediate signal and amplification plots with very similar intensities in both channels 1 and 2 (blue and red curve). Homozygous mutant blood samples, channel 1 (ROX) will show a strong signal and amplification plot (red curve) and channel 2 (Cy5) none or a very low signal and amplification plot (blue curve). So the signals are reversed on homozygous mutant samples.
Here we are detecting the same SNP in the Factor V gene (rs6025; G1691A), which is connected with a higher risk for venous thrombosis, with a fluorescent hybridization probe assay.
Three blood samples with known genotype were used. All the genotypes can clearly be identified. The blue curve shows the specific wild type (homozygous G;G) peak at 61°C. The red curve shows the specific mutant (homozygous A;A) peak at 53°C. A heterozygous case (A;G) will result in both melting curve peaks, respectively at the same specific temperature for wild type and mutant (orange curve).
DirectBlood Genotyping PCR Kit is a novelty on the market. It allows genotyping in real-time and without any previous DNA isolation. You simply save time and money: directly use EDTA blood without any extraction steps.
The DirectBlood Genotyping PCR Kit is optimized to function with a wide range of different SNPs to detect possible nucleotide variations. It has been successfully tested with a variety of hydrolysis probes and hybridization probes.
Description
AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 2,000 bp
Concentration
45.5 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis.
Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.
Cryptosporidium TaqMan RT-PCR Kit, 100 reactions
Cryptosporidium TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM39150 (100 preps) | Cat. TM39110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Cryptosporidium Primer & Probe Mix | 280 μL | 280 μL |
| Cryptosporidium Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
