endo-BCN-PEG12-NHS ester is a aqueous soluble PEG linker containing an NHS ester group and a BCN group. It can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group enable copper free click chemistry with azide-attached molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG12-NHS ester is a aqueous soluble PEG linker containing an NHS ester group and a BCN group. It can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group enable copper free click chemistry with azide-attached molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Kit Contents
| Contents | R401601 | R401602 |
| Purification Times | 1 x 96 | 4 x 96 |
| HiPure RNA Plate | 1 | 4 |
| 1.5ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| RTL Lysis Buffer | 100 ml | 400 ml |
| RNA Binding Buffer* | 30 ml | 120 ml |
| Buffer RW1 | 100 ml | 400 ml |
| Buffer RW2* | 50 ml | 2 x 100 ml |
| RNase Free Water | 20 ml | 80 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.
Available upon request and for R&D use only – Contact Us
The m6A sensitive DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.
The enzyme can also be used for real-time cycling, when adding a suitable dye.
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.
ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.
Originally isolated from Pandalus borealis (Arctic shrimp), it has been purified from a recombinant source since 2010. Unlike other alkaline phosphatases, rSAP can be fully inactivated by a short heat treatment of 15 minutes at 65°C.
This added convenience through complete heat inactivation has made SAP one of the most sold DNA modifying enzymes.
For added flexibility, especially when lyophilisation may be desired, rSAP is also available in a Glycerol FREE format. First launched in 1993, SAP’s unique features continue to make it a valuable tool in various applications.
ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.
