endo-BCN-PEG12-NHS ester is a aqueous soluble PEG linker containing an NHS ester group and a BCN group. It can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group enable copper free click chemistry with azide-attached molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG12-NHS ester is a aqueous soluble PEG linker containing an NHS ester group and a BCN group. It can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group enable copper free click chemistry with azide-attached molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | 96 well Bind Plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR product, enzymatic reaction |
| Sample amount | Appropriate |
| Recovery | ≥80% |
| Elution volume | ≥75μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D212201 | D212202 | D212203 |
| Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
| Buffer GDP | 120 ml | 400 ml | 4 x 400 ml |
| Buffer DW2 | 20 ml | 100 ml | 3 x 100 ml |
| Elution Buffer | 20 ml | 20 ml | 30 ml |
| HiPure DNA Plate | 1 | 4 | 20 |
| 2.2ml Collection Plate | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.8 ml Collection Plate | 1 | 4 | 20 |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Specifications
| Features | Specifications |
| Recommended application | Small amounts of nucleic acid isolation, viral nucleic acid from cell free samples |
| Preservation conditions | Room temperature |
| Stability | Up to 4 years |
| Filter membrane | High quality glass fiber filter GF/F, 2 layers |
| Membrane aperture | 0.7μm |
| Maximum binding yield of plasmid | 30 μg |
| Maximum yield of alcohol mediated Binding | 100 μg |
| Single liquid carrying capacity of column | 900 μl |
| Minimum elution volume | 80 μl |
| Withstand centrifugal force | 5,000 x g |
| Centrifuge | Low speed centrifuge, Swing out Rotor, can placed a height of 6.5cm square, (height of HiPure DNA Plate & 1.6ml Collection Plate: height, 6.2cm) |
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
| CAT.No. | Product Name | Package |
| C13130 | HiPure DNA Plate (2 x GF/F)with 1.6ml Collection Plate | 10/Bag |
| Item No. | Product Name | Membrane type/number of layers | Collection tubes | Plasmid DNA binding capacity (Physical adsorption) | gDNA/RNA binding capacity (Alcohol-mediated adsorption) | Minimum Elution volume | Liquid volume capacity |
| C13010 | HiPure DNA Nano Column | 2 layers GF/F | 2ml without cap | 5μg | 20μg | 10μl | 700μl |
| C13011 | HiPure DNA Micro Column | 3 layers GF/F | 2ml without cap | 10μg | 50μg | 15μl | 700μl |
| C13100 | HiPure DNA Mini Column I | 2 layers GF/B | 2ml without cap | 15μg | 100μg | 30μl | 700μl |
| C13110 | HiPure DNA Mini Column II | 4 layers GF/B | 2ml without cap | 35μg | 200μg | 50μl | 800μl |
| C13111 | HiPure RNA Mini Column | 3 layers GF/B | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
| C13112 | HiPure Viral Mini Column | 3 layers GF/F | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
| C13113 | HiPure CFDNA Mini Column | 3 layers GF/F,1 layer GF/B | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
| C13120 | HiPure DNA Midi Column | 4 layers GF/B | 15ml Centrifuge tube | 125μg | 1mg | 500μl | 4ml |
| C13121 | HiPure DNA Midi Column III | 8 layers GF/B | 15ml Centrifuge tube | 250μg | 1mg | 500μl | 4ml |
| C13122 | HiPure DNA Maxi Column | 4 layers GF/B | 50ml Centrifuge tube | 500μg | 5mg | 1000μl | 20ml |
| C13123 | HiPure DNA Maxi Column III | 8 layers GF/B | 50ml Centrifuge tube | 1mg | 5mg | 1000μl | 20ml |
| C13124 | HiPure DNA Maxi Column C | 8 layers GF/B | 50ml high speed Centrifuge tube | 1mg | 5mg | 700μl | 12ml |
| C13130 | HiPure DNA Plate | 2 layers GF/F | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
| C13131 | HiPure gDNA Plate | 2 layers GF/B | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
Features
1.Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect sleeve , 3 tiers protection,safe and reliable.
7. Rotor is connected to spindle by specialized spring taper sleeve, loading simple and quick, no direction
8. With pre-cooling and get the ideal centrifugation result.
TGL16 Technical Parameter：
| Max. Speed | 16000rpm |
| Max. RCF | 20600×g |
| Max. Capacity | 6×100ml |
| Time Range | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 58 |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (L x W x Hmm) | 610×570×370mm |
| Net Weight(Kg) | 82KG |
| Certificates | TUV CE,ISO 9001,ISO13485 & Calibration report are available |
Matched Rotor for TGL16
| Order No | Rotor | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| G16-1 | Angle rotor | 14000 | 4×8PCR | 12070 |
| G16-2 | Angle rotor | 13000 | 6×8PCR | 16080 |
| G16-3 | Angle rotor | 14000 | 8×8PCR | 13390 |
| G16-4 | Angle rotor | 13000 | 12×8PCR | 17220 |
| G16-5 | Angle Rotor | 15000 | 30×0.5ml | 18510 |
| G16-6 | Angle Rotor | 14000 | 40×0.5ml | 19970 |
| G16-7 | Angle Rotor | 16000 | 12×1.5/2ml | 17940 |
| G16-8 | Angle Rotor | 15000 | 24×1.5/2ml | 20600 |
| G16-9 | Angle Rotor | 13500 | 30×1.5/2ml | 19340 |
| G16-10 | Angle Rotor | 11000 | 48×1.5/2ml | 12840 |
| G16-11 | Angle Rotor | 15000 | 16×5ml | 19350 |
| G16-12 | Angle Rotor | 14000 | 12×7ml | 16370 |
| G16-13 | Angle Rotor | 14000 | 6×10ml | 16460 |
| G16-14 | Angle Rotor | 12000 | 12×10ml | 14510 |
| G16-15 | Angle Rotor | 10000 | 12×15ml | 11840 |
| G16-16 | Angle Rotor | 5000 | 24×15ml | 3080 |
| G16-17 | Angle Rotor | 5000 | 30×15ml | 3830 |
| G16-18 | Angle Rotor | 12000 | 8×20ml | 14510 |
| G16-19 | Angle Rotor | 12000 | 6×30ml | 14000 |
| G16-20 | Angle Rotor | 11000 | 6×50ml | 13480 |
| G16-21 | Angle Rotor | 12000 | 6×70ml | 10810 |
| G16-22 | Angle Rotor | 8000 | 6×100ml | 7280 |
| G16-23 | Angle Rotor | 12000 | 24 pieces capillary vessel | 15800 |
| G16-24 | Microplate rotor | 4000 | 2×3×48 Well | 2300 |
