Introduction

Intended Use

For selective enrichment culture of Listeria monocytogenes in food.

Principle and Interpretation

Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; esculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.

Formulation

Preparation

Suspend 57.4g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃,aseptically add the contents of 1 vial of Fraser Selective Supplement (SR0120) to each 225 mL of base to prepare FB1 solution; Add one reagent (SR0130) to each100 mL of FB2 culture medium to prepare FB2 enrichment solution.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 46~50 hours

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

ISO 11290

Intended Use For selective enrichment culture of Listeria monocytogenes in food. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen ……

Propargyl-PEG4-NHS ester is an amine reactive PEG linker with an alkyne group. Under the copper catalyzation, alkyne group can react with azide-bearing biomolecules to form a stable triazole linkage. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG4-NHS ester is an amine reactive PEG linker with an alkyne group. Under the copper catalyzation, alkyne group can react with azide-bearing biomolecules to form a stable triazole linkage. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.

Details

Specifications

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
• Fast – polydisperse magnetic beads, fast magnetic response and short extraction time
• High purity – the obtained DNA can be directly used for second-generation sequencing, PCR based detection, gene bank, etc.
• Automatic – saving time andlabor and safer

Kit Contents

Storage and Stability

Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage(up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.