Propargyl-PEG13-bromide

Name of Product

HIT – Heparin-Induced Thrombocytopenia Test (20)

Catalog Number

MQHIT 1

Short Info

Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum

This product is only available in Germany!

Method/Platform

lateral flow, immunoassay

Range/Assay Sensivity

Test Principle

IgG antibodies are resposible for the heparin induced thrombocytopenia.

Immobilized anti-human IgG on the membrane of the test unit binds patients IgG-antibodies which are previously captured by the PF4/polyanion-complex which is detected by intensely colored gold nanoparticles.

The presence of PF4/polyanion-complex becomes visible at a colored test line. The surplus of gold particles continues to migrate through the membrane and is captured at the control line by specific antibodies.

0 tests
Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum

Introduction

Intended Use

For selective enrichment culture of Listeria monocytogenes in food.

Principle and Interpretation

Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; esculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.

Formulation

Ingredients	/liter
Enzymatic digest of animal tissues　( Proteose peptone)	5.0g
Enzymatic digest of casein　( Tryptone)	5.0g
Meat extract	5.0g
Yeast extract	5.0g
Sodium chloride	20.0g
Disodium hydrogen phosphate dihydrate	12.0g
Potassium dihydrogen phosphate	1.35g
Aesculin	1.0g
Lithium chloride	3.0g
pH7.2±0.2 at 25°C

Preparation

Suspend 57.4g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃,aseptically add the contents of 1 vial of Fraser Selective Supplement (SR0120) to each 225 mL of base to prepare FB1 solution; Add one reagent (SR0130) to each100 mL of FB2 culture medium to prepare FB2 enrichment solution.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 46~50 hours

Quality control strains	Growth	Characteristics
Listeria monocytogenes ATCC19115 + Escherichia coli ATCC25922 + Enterococcus faecalis ATCC29212	>20 cfu  On PALCAM	Gray to black colony count with black halo
Escherichia coli ATCC25922	< 100 colonies on TSA	Inhibition
Enterococcus faecalis ATCC29212

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

ISO 11290

Intended Use For selective enrichment culture of Listeria monocytogenes in food. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen ……

The Opentrons Thermocycler Module GEN2 is a fully automated on-deck thermocycler, providing hands-free PCR in a 96-well plate format. Compatible with Opentrons hardware and software to allow for fully automated PCR reaction setup and thermocycling on your Opentrons robot. The heated lid and disposable seal fit tightly over the plate, ensuring efficient sample heating and minimal evaporation.

If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.

The Opentrons Thermocycler Module GEN2 is a fully automated on-deck thermocycler, providing hands-free PCR in a 96-well plate format. Compatible with Opentrons hardware and software to allow for fully automated PCR reaction setup and thermocycling on your Opentrons robot. The heated lid and disposable seal fit tightly over the plate, ensuring efficient sample heating and minimal evaporation.

If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.