Bis-propargyl-PEG6 has two propargyl groups which can participate in Click Chemistry reactions to yield a stable triazole linkage with azide compounds; copper is needed as a catalyst. The 6 units of PEG increase the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG6 has two propargyl groups which can participate in Click Chemistry reactions to yield a stable triazole linkage with azide compounds; copper is needed as a catalyst. The 6 units of PEG increase the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Exceptional value for money
Rapid detection of 2019-nCoV
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
| Usage | For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection. |
| Formula (per liter) | Peptone—————10.0g Beef Extract———–3.0g Sodium Chloride—–5.0g Final pH———–7.4 ± 0.2 |
| How to use | 1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121ºC for 15 minutes. 2.Diluted and treated samples. |
| Storage | Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years. |
500g
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots |
| Applications | PCR, qPCR, southern bolt and virus detection, etc |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples |
| Sample amount | Solid tissue: 1-10mg; Anticoagulant blood: 200μl |
| Elution volume | ≥20μl |
| Time per run | 30 – 60 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | D301802 | D301803 |
| Purification Times | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 100 | 500 |
| Buffer ATL | 30 ml | 150 ml |
| Buffer AL | 30 ml | 150 ml |
| Buffer GW1 | 22 ml | 88 ml |
| Buffer GW2 | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer AE | 15 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
