Aminooxy-PEG3-propargyl has a alkyne group and an aminooxy group. The aminooxy group reacts with aldehydes or ketones to form a stable oxime linkages. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
Aminooxy-PEG3-propargyl has a alkyne group and an aminooxy group. The aminooxy group reacts with aldehydes or ketones to form a stable oxime linkages. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
Norgen’s Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components:
Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for epidemiological and population studies.
Norgen’s Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.
Prior to saliva DNA isolation, vortex the Collection Tube containing preserved saliva for 10 seconds and incubate at 55°C for one hour. Saliva DNA can now be isolated from the preserved saliva samples using any commercially available method, including Norgen’s Saliva DNA Isolation Kit (Cat. RU45400), Norgen’s Saliva DNA Isolation 96-Well Kit (Cat. RU35200) and Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) (Cat. RU55400, RU62900). Also, Norgen’s Saliva DNA Isolation Reagent Kit (up to 4 mL) (Cat. RU35720) allows for DNA isolation from the preserved saliva samples using an alcohol precipitation method. The purified DNA is of the highest quality and can be used in a number of downstream applications including PCR, NGS and microarray analysis.
Figure 1 / 3
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| Kit Specifications | |
| Volume of Saliva Collected | 2 mL |
| Volume of Saliva-Preservative Mix | 4 mL |
| Preservation Temperature | Room Temperature |
| Preservation time | Over 2 years at room temperature |
Shelf Life and Handling
| Kit Components | Cat. RU49000 (50 Devices) |
|---|---|
| Individual Saliva DNA Collection and Preservation Devices | 50 |
| Donor Procedure Flowchart | 1 |
| Product Insert | 1 |
| Individual Saliva DNA Collection and Preservation Device Contents | |
| Saliva Collection Funnel and Collection Tube | 1 |
| Collection Tube Cap | 1 |
| Preservative Ampoule | 1 |
| Donor Instructions | 1 |
Mycoplasma is the smallest and simplest prokaryotic organism. There is a risk of mycoplasma contamination during cell culture, and mycoplasma contamination of cells has become a common problem worldwide.
Mycoplasma contamination may seriously affect the state of cells, change the gene expression and metabolic characteristics of cells, lead to slow cell growth, abnormal differentiation and death, and seriously affect cell function.
Bacteria, yeast or mold contamination in cell culture can be seen under an optical microscope, but mycoplasma contamination is usually not visible under an optical microscope and must be detected by specific detection methods.
Common methods for detecting mycoplasma contamination include mycoplasma isolation and culture, special biochemical tests such as ELISA and luminescence, and DNA fluorescent staining detection. Among the above detection methods, most of the operation steps are relatively cumbersome, the sensitivity is not high, the mycoplasma types cannot be distinguished, special instruments are required, or the time required is long. The qPCR method is relatively simple and convenient to operate, and the results can be obtained in 2 hours.
• Provide results in less than 2 hours.
Good negative and positive controls.
• No live mycoplasma required: The MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require the use of live mycoplasma for validation
• Can be used with gDNA, inactivated mycoplasma, or live mycoplasma
• Can be used as an alternative to the standard 28-day culture test
• Can be used in conjunction with culture-based methods to provide preliminary results while awaiting the 28-day test results
The MycoSEQ Plus Mycoplasma Detection Kit is part of an integrated workflow for adventitious drug, impurity, and contaminant detection during biopharmaceutical manufacturing. The entire workflow includes the sample preparation kit, which provides a manual sample preparation method, which together with the qPCR analysis can provide results in 2 hours. (Please note that complex matrices may require additional upfront processing steps as described in the various available protocols.)
• Mycoplasma qPCR MIX, store at -15°C to -25°C, store at 2°C to 8°C after first use, protected from light.
• Mycoplasma Positive Control, -15°C to -25°C.
• Mycoplasma Negative Control, store at -15°C to -25°C, store at 2°C to 8°C after first use.
Note: Price not include shipment & duty, contact us to get full quote.
DuckyBio’s Mycoplasma PCR Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for detecting mycoplasma contamination in biological materials such as cultured cells. Designed and tested using criteria often applied toward rapid mycoplasma detection in biotherapeutic manufacturing cell culture lot release, the MycoSEQ Plus kit enables results that meet or exceed guidance on sensitivity and specificity expectations as described in European Pharmacopoeia (E.P. 2.6.7, 2007), US Pharmacopoeia (US63) and Japanese Pharmacopoeia. When used with a suitable sample preparation method, the MycoSEQ Plus kit can detect less than 5 CFU/mL.
K-CellG3
180 / 360 assays per kit
| Content: | 180 / 360 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | endo-Cellulase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.05 U/mL |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | ~ 20 min |
| Application examples: | Fermentation broths, industrial enzyme preparations and biofuels research. |
| Method recognition: | Novel method |
This product has been discontinued (read more).
The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Please note that a new assay kit (K-CellG5) is now available for the measurement of endo-cellulase. The CellG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases. In addition, the exchange of the benzylidene blocking group in CellG3 for 3-keto-butylidene in CellG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay. As DMSO is known to inhibit certain cellulases, this is another benefit in using CellG5. Megazyme now recommends the use of K-CellG5 for all assays for the measurement of endo-cellulase.
Browse more cellulase and other enzyme activity assay kits.
The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
