Aminooxy-PEG3-propargyl has a alkyne group and an aminooxy group. The aminooxy group reacts with aldehydes or ketones to form a stable oxime linkages. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
Aminooxy-PEG3-propargyl has a alkyne group and an aminooxy group. The aminooxy group reacts with aldehydes or ketones to form a stable oxime linkages. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard.
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Storage Conditions
Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
| Component | Cat. 58900 (48 reactions) |
|---|---|
| microScript microRNA Enzyme Mix | 20 μL |
| 2x microScript Reverse Transcriptase Reaction Mix | 0.5 mL |
| 2X Real-Time PCR Master Mix | 1 mL |
| RNA Quantification Primer Set Mix | 200 μL |
| Quantified RNA Standard 50 pg/μL | 2 x 60 μL |
| Nuclease-Free Water | 3 x 1.25 mL |
| Product Insert | 1 |
Non-woven gas permeable seal that limits evaporation. The seal is peelable, pierceable; suitable for cell culture and seed & insect storage too.
For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method.
MUG permits the rapid detection of Escherichia coli when the medium is observed for fluorescence using a UV light. MUG is hydrolyzed by the enzyme β-glucuronidase which is produced by E. coli to yield a fluorescent end product 4- methylumbelliferone. Trypticase provides the essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentative action. The bile salts inhibit gram-positive bacteria especially the Bacillus species and faecal Streptococci.
| Ingredients | /liter |
| Trypticase or tryptose | 20 g |
| Bile salts No. 3 | 1.5 g |
| Lactose | 5 g |
| K2HPO4 | 4 g |
| KH2PO4 | 1.5 g |
| NaCl | 5 g |
| 4-methylumbelliferyl-β-D-glucuronide (MUG) | 50mg |
| pH6.9±0.2 at 25°C | |
Weigh 37g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until
completely dissolved, sterilize at 115℃ for 20min, cool to room temperature and set aside.
The following quality control strains were inoculated and cultured at 44.5℃±0.5℃ for 24h. The results are as follows:
| Quality control strains | Growth |
| Escherichia coli ATCC25922 | Blue-white fluorescence is produced under 366nm UV light |
| Salmonella typhimurium ATCC14028 | No fluorescence under 366nm UV light |
| Enterococcus faecalis ATCC29212 | Clear, no fluorescence |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method. Principle and Interpretation MUG permits the rapid detec……
