Diazo Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. The hydrophilic spacer arm provides better solubility to the labeled molecules in aqueous media. Diazo allows efficient release of captured biotinylated molecules from streptavidin using sodium dithionite (Na2S2O4). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Diazo Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. The hydrophilic spacer arm provides better solubility to the labeled molecules in aqueous media. Diazo allows efficient release of captured biotinylated molecules from streptavidin using sodium dithionite (Na2S2O4). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TD5A Low Speed Centrifuge
Features:
1. Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.
2. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.
3. Automatic lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
4. Adapters are available by experiment requirements.
5. 3 tiers protection steel cover, safe and reliable.
6. TUV CE EMC certificate.
TD5A Technical Parameter：
Matched Rotor for TD5A
| Order NO. | Rotor Type | Max speed(r/min) | Volume (ml) | Max RCF(*g) |
| 5A-1 | Swing rotor (round cup) | 4000 | 4×300ml | 3180 |
| 5A-2 | Swing rotor (square cup) | 4000 | 4×300ml | 3150 |
| 5A-3 | Swing rotor | 4000 | 4×24×7ml | 3150 |
| 4000 | 4×24×5ml | 2750 | ||
| 4000 | 4×18×10ml | 3140 | ||
| 5A-4 | Microplate rotor | 4000 | 2×4×96 well | 2910 |
| 5A-5 | Microplate rotor | 4000 | 2×4×48 well | 2300 |
| 5A-6 | Swing rotor | 5000 | 4×1×50ml | 4730 |
| 5000 | 4×1×100ml | 4730 | ||
| 5A-7 | Swing rotor | 4000 | 4×2×50ml | 3200 |
| 4000 | 4×2×100ml | 3020 | ||
| 4000 | 4×4×15ml | 3200 | ||
| 4000 | 4×6×15ml | 3200 | ||
| 4000 | 4×8×15ml | 3200 | ||
| 4000 | 4×12×5ml vacuum tube | 2480 | ||
| 4000 | 4×12×7ml vacuum tube | 2760 | ||
| 4000 | 4×12×10ml vacuum tube | 2790 | ||
| 4000 | 4×8×10/7/5ml vacuum tube | 2790 | ||
| 4000 | 4×6×10/7/5ml vacuum tube | 2790 | ||
| 4000 | 4×4×10/7/5ml vacuum tube | 2790 | ||
| 5A-8 | Fixed Rotor | 5000 | 6×15ml | 2540 |
| 5A-9 | Fixed Rotor | 5000 | 12×15ml | 3080 |
| 5A-10 | Fixed Rotor | 5000 | 24×15ml | 3500 |
| 5A-11 | Fixed Rotor | 5000 | 30×15ml | 3830 |
| 5A-12 | Fixed Rotor | 5000 | 4×50ml | 2520 |
| 5A-13 | Fixed Rotor | 5000 | 6×50ml | 2850 |
| 5A-14 | Fixed Rotor | 5000 | 12×50ml | 3860 |
| 5A-15 | Fixed Rotor | 4000 | 24×50ml | 2970 |
| 5A-16 | Fixed Rotor | 5000 | 4×100ml | 2630 |
| 5A-17 | Fixed Rotor | 5000 | 6×100ml | 3130 |
| 5A-18 | Fixed Rotor | 4000 | 12×100ml | 2970 |
TD5A centrifuge Max Capacity 4x300ml swing out rotor, max speed 5000rpm, can hold 96 vacuum tubes
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
SNPsig® EscapePLEX™ SARS-CoV-2 also incorporates the two gene (ORF1ab and M Gene) assay to provide a confirmatory detection of SARS-CoV-2.
DETECTION PROFILE: The E484K, K417N, K417T and P681R mutations which are found in Beta, Gamma, Delta and Delta Plus Variants of Concern
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions
The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.
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DNA size selection is a selective capture of DNA fragments of a specific range of size for next-generation sequencing (NGS) library preparations, PCR, ChIP assay, DNA ligations, endonuclease digestions, adapter removal, and other genomics and molecular biology applications. DNA size selection is preferred after NGS library prep in most of the cases. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads technology uses paramagnetic particles, also known as SPRI (Solid Phase Reversible Immobilization) beads, to bind DNA reversibly and selectively. DNA fragments can be size-selected and purified by changing the properties of the magnetic beads or SPRI beads. The magnetic beads can easily separate the beads-binding DNA from the contaminants and unwanted components in the samples. The samples after DNA size selection are free of contaminants such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. Our proprietary magnetic beads reagents improve yield, selectivity, and reproducibility.
Specific DNA fragments at a certain length range can be purified simply using magnetic separation with different beads components, avoiding tedious and time-consuming gel extraction and column-based purification. The magnetic beads method is popular for common DNA size selection, including library size selection. The first beads-binding step, referred to as the right-side clean-up, removes large DNA fragments. The large DNA fragments are bound to the beads and are discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-binding, referred to as the left-side clean-up. The double-size selected DNA fragments are eluted after ethanol rinsing.
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A single clean-up is needed for DNA size selection with large fragments. In this case, only the large DNA fragments are bound to the beads. The selected larger DNA fragments are eluted after ethanol rinsing.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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