PC Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. PEG spacer provides better solubility to the labeled molecules in aqueous media. Captured biomolecules can be efficiently photoreleased using near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
PC Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. PEG spacer provides better solubility to the labeled molecules in aqueous media. Captured biomolecules can be efficiently photoreleased using near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG12-DBCO is a long chain click chemistry PEG reagents. The DBCO group can react with azides via a copper-free Click Chemistry reaction to form a stable triazole. This reagent can be applied to situations when copper is a concern for DBCO-activated PEGylation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG12-DBCO is a long chain click chemistry PEG reagents. The DBCO group can react with azides via a copper-free Click Chemistry reaction to form a stable triazole. This reagent can be applied to situations when copper is a concern for DBCO-activated PEGylation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.
Stool DNA Isolation Kit (Spin Column)
Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation 96-Well Kit (High Throughput)
Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation Kit (Magnetic Bead System)
Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization).
Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system.
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| Kit Specifications | |
| Maximum Stool Input | 200 mg (fresh/frozen stool) or 400 μL (preserved stool) |
| Type of Stool Processed | Frozen, fresh or preserved stool |
| Format | Spin Column |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Elution Volume | 50 μL |
| Time to Complete 10 Purifications | 30 minutes |
| Applications | PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis. |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 27600 (50 preps) | Cat. 65600 (192 preps) | Cat. 55700 (50 preps) | Cat. 63100 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer L | 60 mL | 2 x 105 mL | 60 mL | 3 x 60 mL 1 x 30 mL |
| Lysis Additive A | 6 mL | 25 mL | 6 mL | 25 mL |
| Binding Buffer I | 7 mL | 25 mL | 7 mL | 25 mL |
| Binding Buffer C | 30 mL | 110 mL | – | – |
| Wash Solution A | 18 mL | 2 x 38 mL | – | – |
| Magnetic Bead Suspension | – | – | 1.1 mL | 4 x 1.1 mL |
| Solution WN | – | – | 18 mL | 55 mL |
| Elution Buffer B | 8 mL | 30 mL | 8 mL | 30 mL |
| Bead Tubes | 50 | 200 | 50 | 196 |
| Mini Spin Columns | 50 | – | – | – |
| 96-Well Plate | – | 2 | – | 2 |
| Adhesive Tape | – | 4 | – | 2 |
| Collection Tubes | 50 | – | – | – |
| 96-Well Collection Plate | – | 2 | – | – |
| Elution Tubes (1.7 mL) | 50 | – | 50 | – |
| 96-Well Elution Plate | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA/DNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases H, the gold tagged RNA/DNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase H is detected). When RNases H is present or added, however, the RNA strand of the RNA/DNA substrate is cleaved, and the Biotin tagged 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases H activity and is a perfect tool for monitoring unit activity or residual RNAse H activity.
RNase H activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase H activity in enzyme preparations and determination of RNase H unit activity.
