Propargyl-PEG5-t-butyl ester has one of the functional groups protected by t-butyl group. The protection can be removed under acidic conditions. The propargyl group forms triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The PEG units help improving the water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG5-t-butyl ester has one of the functional groups protected by t-butyl group. The protection can be removed under acidic conditions. The propargyl group forms triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The PEG units help improving the water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-NHS ester contains a propargyl group and an NHS ester. As an amine reactive reagent, this product can be used for derivatizing peptides, antibodies, amine coated surfaces etc. In the presence of copper catalyst, propargyl group reacts with azide to yield a stable triazole linkage. The PEG units help increase the hydrophilicity of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-NHS ester contains a propargyl group and an NHS ester. As an amine reactive reagent, this product can be used for derivatizing peptides, antibodies, amine coated surfaces etc. In the presence of copper catalyst, propargyl group reacts with azide to yield a stable triazole linkage. The PEG units help increase the hydrophilicity of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Primerdesign Ltd COVID-19 genesig® Real-Time PCR assay is a real-time RT-PCR assay intended for the qualitative detection of nucleic acid from SARS-CoV-2 in oropharyngeal swab specimens from patients suspected of COVID-19 by their healthcare provider. Testing is limited to laboratories certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a, to perform high complexity tests.
*FDA clinical study. See Instructions for Use for additional clinical data.
FOR US ONLY
Rapid detection and exclusive to the COVID-19 strain
Does not detect other related coronavirus strains
High priming efficiency
Accurate controls to confirm extraction, and assay validity
Lyophilised components for ambient shipping
Highly specific detection profile
This test has been authorized by FDA under an EUA for use by authorized laboratories.
Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit
For sample input volumes ranging from 5 mL to 10 mL.
Cell Culture Media Exosome Purification and RNA Isolation Midi Kit
For sample input volumes ranging from 10 mL to 20 mL.
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit
For sample input volumes ranging from 20 mL to 35 mL.
Figure 1 / 3
Click for expanded view
| Kit Specifications | |
| Sample Type | Cell-Free Cell Culture Media |
| Sample Volume Range | 20 mL to 35 mL |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 40 to 45 minutes |
| Average Yields* | Variable depending on specimen |
* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Cat. 60700 (50 preps) | Cat. 60800 (25 preps) | Cat. 60900 (15 preps) |
|---|---|---|---|
| Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
| ExoC Buffer | 1.5 mL | 1.5 mL | 1.5 mL |
| ExoR Buffer | 12 mL | 12 mL | 12 mL |
| Lysis Buffer A | 20 mL | 20 mL | 20 mL |
| Lysis Additive B | 2 mL | 2 mL | 2 mL |
| Wash Solution A | 18 mL | 18 mL | 18 mL |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Mini Filter Spin Column | 50 | 25 | 15 |
| Mini Spin Columns | 50 | 25 | 15 |
| Collection Tubes | 50 | 25 | 15 |
| Elution tubes (1.7 mL) | 100 | 50 | 30 |
| Product Insert | 1 | 1 | 1 |
