Propargyl-PEG4-methane is a crosslinking reagent that can react with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-methane is a crosslinking reagent that can react with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Character of Yingtai laboratory scale freeze dryer
Small size, and light weight
Compact construction of tabletop freeze dryer
Suitable for small samples drying
Advantage of Yingtai 3kgs smallest lab scale freeze dryer
3kg water capture capacity, which can meet the needs of general laboratory freeze-drying capacity
-70°C ultra-low temperature cold trap, which can capture conventional organic solvents
Direct-capture coil cold trap with higher efficiency and better ice-catching effect
The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry organic solvents
Large flat flange type silicone seal, effectively guarantee the vacuum tightness, no need to apply any auxiliary sealing silicone grease forever
One-button operation, the host and the vacuum pump are linked, no manual operation required
The Pirani vacuum sensor with automatic temperature compensation can accurately detect the vacuum degree in the chamber at each stage
Removable sample shelf with special holes for centrifuge tubes, making it easier to place and adjust the height
Various types of chambers and T-frame configurations can fully meet the user’s sample placement
Vacuum pumps and sample placement chambers can be freely combined according to user needs, making the configuration more flexible and convenient
Techinical parameter
| Item No | BD1-3 | BD1-6 | BD2-6 |
| 24/h water capture capacity | 3kg | 6kg | 6kg |
| Cold trap temperature | -70℃ | -70℃ | -90℃ |
| Cold trap volume | 5L | 10L | 10L |
| Freeze dried chamber | High permeability organic glass/multi manifold external valve/stainless steel chamber | ||
| Sample shelf | φ 200mm stainless steel with 3-5 layers available | ●Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional | |
| vacuum pump | Standard configuration | ||
| Oil mist filter | Extreme vacuum 4 * 10-4mbar,with a pumping capacity of 78L~200L/optional pump of various types | ||
| Vacuum pump connection pipe | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | ||
| anti-corrosive treatment | Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents | ||
| External valve | 6/optional | 1-48 pieces/optional | |
| Eutectic point detection system | Support/Optional | ||
| Data output analysis | Support/Optional | ||
| Power | 0.6kw | 1.1kw | 0.5kw |
Norgen’s EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.
EXTRAClean Cell-Free Circulating RNA Purification Mini Kit:
For sample volumes ranging from 250 μL to 2 mL
EXTRAClean Cell-Free Circulating RNA Purification Midi Kit:
For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL
EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit:
For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
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Click for expanded view
| Kit Specifications | |
|---|---|
| Sample Type | Fresh or frozen urine |
| Sample Volume Range | 10 to 30 mL |
| Minimum Elution Volume | 50 μL |
| Maximum Elution Volume | 100 μL |
| Time to Complete 10 Purifications | 40–50 minutes |
| Size of RNA Purified | All sizes, including miRNA and small RNA (<200 nt) |
| Average Yields ¥ | Variable depending on specimen |
¥Please check page 5 of the protocol for average urine yields and common RNA quantification methods.
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Mini (Cat. 72900) | Midi (Cat. 72910) | Maxi (Cat. 72920) |
|---|---|---|---|
| Binding Solution K | 25 mL | 75 mL | 1 x 75 mL 1 x 25 mL |
| Lysis Buffer A | 30 mL | 20 mL | 20 mL |
| Wash Solution A | 18 mL | 18 mL | 18 mL |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Collection Tubes | 50 | 20 | 10 |
| Elution Tubes (1.7 mL) | 50 | 20 | 10 |
| EXTRAClean Mini Spin Columns | 50 | 20 | 10 |
| EXTRAClean Midi Spin Columns | – | 20 | – |
| EXTRAClean Maxi Spin Columns | – | – | 10 |
| Product Insert | 1 | 1 | 1 |
