Propargyl-PEG8-methane is crosslinker with a propargyl group. It enables the formation of triazole linkage with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG8-methane is crosslinker with a propargyl group. It enables the formation of triazole linkage with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bile Salt Export Pump (BSEP) is a member of the ATP-binding cassette (ABC) transporters, which mediates the transport of bile acid, taurocholate and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus. BSEP is associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) and benign recurrent intrahepatic cholestasis type 2 (BRIC2). PFIC2 caused by mutations in the BSEP gene increases the risk of hepatocellular carcinoma in early life.
Sample type purification kit guide
The 16S V3-V4 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V3-V4 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V3-V4 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70400 (24 preps) | Cat. 70410, 70420, 70430, 70440 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V3-V4 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
DD5 Low Speed 5000rpm Swing rotor 4x800ml lab Centrifuge Adapters for 5/10/15/50ml tubes.
DD5 Low Speed Large Capacity Centrifuge Application:
DD5 Lab centrifuge widely used in the fields of pharmaceutical factory and laboratory, can hold swing out rotors and angle rotors both.
DD5 Low Speed Large Capacity Centrifuge Features:
1. Brushless frequency motor which in great torque, free maintenance, no powder pollution, quick in speed up and down.
2. Digital display which indicates the program, speed, time, RCF and temperature.
3. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration/deceleration for your choice.
4. There are kinds of rotors for your choice.
5. Electric lid lock, compact design, super speed and imbalance protection.
6. The centrifuge body is made of high-quality steel, safe and reliable.
DD5 Low Speed Laboratory Centrifuge Technical Parameters:
| Max. Speed | 5000rpm |
| Max.RCF | 4730xg |
| Max.Capacity | 4x800ml |
| Timer | 0~99mins |
| RPM/RCF Convert | Yes |
| Noise(DB) | ≤58 |
| Acc/Dec | 10 Rates |
| Speed Accuracy | ±20rpm |
| Voltage(V/HZ) | AC 220V/110V 50HZ/60HZ |
| Dimension(LxWxH mm) | 720x540x830 |
| Net Weight(Kg) | 108kg |
| Certificates | CE,ISO |
| Warranty | 1 Year |
DD5 Low Speed Laboratory Centrifuge Matched Rotors:
| Order No. | Rotor type | Max.Speed (rpm) | Max.Volume (ml) | Max.RCF (xg) |
| No30671 | Swing out rotor | 4,000 | 4x800ml(round cup) | 3,450 |
| No30679 | Swing out rotor | 4,000 | 4x500ml(square cup) | 3,450 |
| No30696 | Swing out rotor | 4,000 | 4x500ml(round cup) | 3,530 |
| No31377 | Swing out rotor | 5,000 | 4x50ml | 4,730 |
| 4x100ml | 4,730 | |||
| No30592 | Swing out rotor | 4,000 | 4x30x7ml | 3,140 |
| 4x40x7ml | 3,270 | |||
| 4x18x10ml | 3,140 | |||
| No31491 | Mircoplater rotor | 4,000 | 2x4x96well | 2,910 |
| No31494 | Microplater rotor | 4,000 | 4x4x96well | 2,940 |
| No30627 | Angle rotor | 5,000 | 30x15ml | 3,830 |
| No30641 | Angle rotor | 5,000 | 12x50ml | 3,860 |
| No30642 | Angle rotor | 4,000 | 24x50ml | 2,970 |
| No30614 | Angle rotor | 5,000 | 6x100ml | 3,130 |
| No30643 | Angle rotor | 4,000 | 12x100ml | 2,970 |
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