APN-C3-PEG4-alkyne is a heterobifunctional linker containing an APN moiety with exquisite chemoselectivity for cysteine and an alkyne group. The superior stability of APN-cysteine conjugates in aqueous media, human plasma, and living cells makes this new thiol-click reaction a promising methodology for applications in bioconjugation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
APN-C3-PEG4-alkyne is a heterobifunctional linker containing an APN moiety with exquisite chemoselectivity for cysteine and an alkyne group. The superior stability of APN-cysteine conjugates in aqueous media, human plasma, and living cells makes this new thiol-click reaction a promising methodology for applications in bioconjugation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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| Kit Specifications | |
| Maximum Binding Capacity Per Column | Up to 50 μg RNA |
| Maximum Loading Volume Per Spin Column | 650 μL |
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications | 25 minutes |
| Maximum Amount of Starting Material | |
| Liver Tissue | 20 mg |
| Heart Tissue | 5 mg |
| Kidney Tissue | 20 mg |
| Brain Tissue | 25 mg |
| Lung Tissue | 20 mg |
| Spleen Tissue | 20 mg |
| Whole Blood | 100 µL |
| Hela Cells | 3 x 106 |
| CHO | 3 x 106 |
| Yeast | 1 x 108 cells |
| E.coli | 1 x 109 CFU/mL |
| Bacteria | 1 x 109 cells |
| Plasma/Serum | 200 µL |
| Fungi | 50 mg |
| Plant Tissue | 50 mg |
| Viruses | ≤100 µL of viral suspension |
| Average Yield | |
| HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg | |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 48300 (50 preps) | Cat. 48400 (100 preps) |
|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL |
| RNA Purification Columns | 50 | 100 |
| Genomic DNA Removal Columns | 50 | 100 |
| Collection Tubes | 100 | 200 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
TDL5B refrigerated low speed centrifuge
Technical Parameters
| Max speed： | 5000rpm | Max RCF： | 4730xg |
| Max volume | 4x800ml | Noise： | ≤65dBA |
| Timer | 1～99h59min | Net weight： | 125 Kg |
| Dimension(HxDxW)： | 430×688×715mm | Power supply | AC 220V 50HZ 15A |
| Temperature range | -20℃~40℃ | Temperature accuracy | ±1℃ |
| Speed accuracy： | ±20rpm | Package | wooden box |
Matched Rotors for TDL5B
| Order No. | Model | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| No.30671 | Swing out rotor | 4000 | 4x800ml (round cup) | 3450 |
| Adapters for 4x800ml (round cup) swing rotor for 5ml, 7ml, 10ml, 15ml, 50ml, 100ml, 250ml, 500ml tubes | ||||
| No.30696 | Swing out rotor | 4000 | 4x500ml (square cup) | 3310 |
| No.31277 | Swing out rotor body | |||
| Matched Buckets for the swing out rotor body | 5000 | 4x50ml | 4420 | |
| 4x100ml | ||||
| 4000 | 8x50ml | 2950 | ||
| 16x15ml | ||||
| 24x15ml | ||||
| 4000 | 24×10/7ml vacuum tube | 2580 | ||
| 16x10ml vacuum tube | ||||
| Order No. | Model | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| No.31377 | Matched Buckets for the swing out rotor body | 5000 | 32x10ml vacuum tube | 2580 |
| 32x7ml vacuum tube adapter | ||||
| 32x5ml vacuum tube adapter | ||||
| 24x10ml vacuum tube | 2580 | |||
| 24x7ml vacuum tube adapter | ||||
| 24x5ml vacuum tube adapter | ||||
| 16x10ml vacuum tube | 2580 | |||
| 16x7ml vacuum tube adapter | ||||
| 16x5ml vacuum tube ada |
RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.
RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.
– half-life at 95°C of >40 min.
– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (<57°C)
– facilitates “zero-step” RT-PCRs directly from RNA templates (without an isothermal reverse transcription step), as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step.
– allows reverse transcription reactions at high temperatures, thus minimizing the problems encountered with strong secondary structures in RNA that melt at elevated temperatures.
– RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes
Please note: Due to the thermostable nature of the enzyme, it is recommended to design very high melting primers and probes (>65°C). See more details in FAQ.
For research use and further manufacturing.
In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.
RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.
– half-life at 95°C of >40 min.
