endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Legionella sp. TaqMan PCR Kit Dx, 24 reactions
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Storage Conditions
The Legionella sp. TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. DxTM64400 (24 rxns) |
|---|---|
| MDx TaqMan 2X PCR Master Mix | 550 μL |
| Legionella sp. Primer & Probe Mix Dx | 2 x 70 μL |
| Legionella sp. Positive Control Dx – 200,000 copies/μL | 50 μL |
| Nuclease-Free Water | 2 x 1.25 mL |
| Product Insert | 1 |
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA and miRNA from cell and tissue without MagZol reagent |
| Applications | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Cell, Animal tissue, Plant tissue |
| Sample amount | Cells: ≤ 5 x 10^6, Animal tissue:<10mg |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.
Advantages
Kit Contents
| Contents | R431102 | R431103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Proteinase K | 48 mg | 240 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer RLC | 40 ml | 200 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
