endo-BCN-PEG2-alcohol

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V3-V5 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V3-V5 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s 16S V3-V5Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Step	Component	Cat. 70500 (24 preps)	Cat. 70510, 70520, 70530, 70540 (96 preps)
Amplicon PCR (PCR 1)	MGX Master Mix	330 µL	1,320 µL
	16S V3-V5 Primer Mix	70 µL	280 µL
Index PCR (PCR 2)	Indexing Master Mix	660 µL	2 x 1,320 µL
	N7xx Index Primer	50 µL	50 µL
	S5xx Index Primer	70 µL	70 µL
PCR Clean-Up	Resuspension Buffer	2 x 1,250 µL	2 x 5,000 µL
	Nuclease-free water	1,250 µL	1 x 6,000 µL

A handheld 8-tube screw cap tube capper/decapper. Securely seal or unscrew an 8-cap row in just 10 seconds, or an entire rack of 96 tubes in 2 minutes.

About

8-Channel Handheld Capper/Decapper – Saving you Time and Effort

The 8-Channel Handheld Capper/Decapper  is a versatile tool designed to increase throughput and save you time and effort. Capable of consistently capping and de-capping multiple tubes simultaneously, this innovative device ensures smooth and efficient lab operations. Its lightweight, ergonomic design reduces operator strain and seamlessly transitions from manual to automated systems, making it an indispensable addition to any laboratory.

Speed and Efficiency

With the ability to securely seal an 8-cap row in just 10 seconds or an entire rack of 96 tubes in 2 minutes, the 8-Channel Handheld Capper/Decapper significantly enhances manual processing throughput. The device guarantees consistent sealing across each tube. Its automatic column-type cap removal and capping performance streamline workflows, ensuring quick and reliable operation. This not only saves valuable time but also reduces the risk of repetitive strain injuries for lab personnel.

Ergonomic and Portable

Designed with user comfort in mind, the 8-Channel Handheld Capper/Decapper is lightweight and ergonomically crafted for effortless screw cap processing. It’s neat, compact design and low-force, dual-button cap ejection system makes it ideal for various laboratory environments. Its included storage stand ensures secure and convenient storage and when not in use.

Convenient Charging and Compatibility

The tool features magnetic contact charging, offering long battery life and fast charging times. Ready to use whenever you are, it efficiently caps, de-caps, or recaps tubes by column or row. Interchangeable cap driver cassettes allow for easy processing of different screw cap tube types and SBS rack formats.

Request a Demo or Speak to an Expert

Experience the efficiency and convenience of the 8-Channel Handheld Capper/Decapper in your lab. Visit our website to request a demo or speak to one of our experts to learn more about how this innovative tool can enhance your laboratory’s operations.