Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

The Primerdesign™ genesig® Kit for Enterocytozoon bieneusi (E. bieneusi) genomes is designed for the in vitro quantification of E. bieneusi genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the E. bieneusi genome. The primers and probe sequences in this kit have 100% homology with a broad range of E. bieneusi sequences based on a comprehensive bioinformatics analysis.

Flex Prep offers an affordable entry point into automation, combining easy-to-install hardware with an intuitive walk-up-and-operate interface

Flex Prep empowers users to start with semi-automated, routine pipetting and seamlessly scale up to full automation as their needs grow. Compatible with additional Opentrons Flex Pipettes and Modules for scaling up to full automation.

Prices listed here are valid in the US and select other territories. Please get in touch with your local team for pricing for your territory.

Flex Prep offers an affordable entry point into automation, combining easy-to-install hardware with an intuitive walk-up-and-operate interface

Flex Prep empowers users to start with semi-automated, routine pipetting and seamlessly scale up to full automation as their needs grow. Compatible with additional Opentrons Flex Pipettes and Modules for scaling up to full automation.

Prices listed here are valid in the US and select other territories. Please get in touch with your local team for pricing for your territory.

Introduction

Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 100-150mg stool sample
Applications	RT-PCR, Northern hybridization and other experiments
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Stool
Sample amount	100-150 mg
Elution volume	≥30μl
Time per run	≤50 minutes
Liquid carrying volume per column	100µg
Binding yield of column	800µl

Principle

The HiPure silica gel column uses a high binding ability glass fiber filter membrane as the substrate. Under the condition of high concentration of ionizing agent (such as Guanidinium chloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonding and electrostatic and other physical factors, while protein or other impurities are not adsorbed and removed. The filter membrane that has adsorbed nucleic acids is washed to remove proteins and salts. Finally, low salt buffer solution (such as Buffer TE) or water can be used to wash out the nucleic acids adsorbed on the filter membrane. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The stool samples are homogenized in the lysis solution, further lysed in a high-temperature water bath, and RNA is released into the lysis solution. Chloroform extraction removes genomic DNA and impurities, transfer the supernatant to an alcohol free binding solution, purify RNA through a column, and finally elute RNA with RNase Free Water. The purified RNA can be directly used for experiments such as PCR, Southern hybridization, and enzyme digestion.

Advantages

• High purity – unique adsorbent for more efficient removal of inhibitors
• High concentration – maximum extraction of total RNA from stool samples
• Sensitive – RNA can be purified at the level of PG

Kit Contents

Contents	R418502	R418503
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
Glass Beads (0.1~0.6mm)	30 g	150 g
Buffer SPL	30 ml	140 ml
Buffer PHC	30 ml	140 ml
Buffer GRP	60 ml	250 ml
Buffer RW1	50 ml	250 ml
Buffer RW2 *	20 ml	2 x 50 ml
RNase Free Water	15 ml	30 ml

Storage and Stability

The kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions. At low temperatures, Buffer SPL may form precipitates, dissolve it by 55°C water bath. After receiving the product, Buffer PHC should be stored at 2-8°C.

Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.