endo-BCN-PEG8-amine is a PEG linker featuring a BCN and a primary amine. The BCN group is a click chemistry handle which quickly reacts with azides while the primary amine can be used to form amides with carboxylic acids in the presence of coupling reagents such as HATU or EDC.
endo-BCN-PEG8-amine is a PEG linker featuring a BCN and a primary amine. The BCN group is a click chemistry handle which quickly reacts with azides while the primary amine can be used to form amides with carboxylic acids in the presence of coupling reagents such as HATU or EDC.
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
1. Urine Preservative Single Dose Ampules
With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.
2. Urine Collection and Preservation Tubes
Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:
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Select Urine Tested with the Norgen Urine Collection and Preservation Tubes
Human
Mouse
Lynx
Wolf
Urine collected from snow
Available Sizes and Formats
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
| Kit Components | Cat. 18118 (50 tubes) | Cat. 18122 (50 tubes) | Cat. 18113 (50 tubes) | Cat. 18126 (50 tubes) | Cat. 18129 (1 cup) |
|---|---|---|---|---|---|
| Urine Collection and Preservation Tubes (5 cc) | 50 | – | – | – | – |
| Urine Collection and Preservation Tubes (15 cc) | – | 50 | – | – | – |
| Urine Collection and Preservation Tubes (50 cc) | – | – | 50 | – | – |
| Urine Preservative Single Dose | – | – | – | 50 | – |
| Urine Collection and Preservation Cup (120 cc) | – | – | – | – | 1 |
| ID Labels | 50 | – | – | 50 | – |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
Intended Use
For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials
Principle and Interpretation
Enzymatic digest of casein and enzymatic digest of animal tissue provides nitrogen source, vitamins and growth factors; sodium chloride maintains balanced osmoticpressure; sorbitol is a fermentable sugar; No. 3 bile salt and crystal violet inhibit Gram-positive bacteria, potassiumtellurite inhibits non-o157 Escherichia coli, and cefixime inhibits Proteus; neutral red is a pH indicator; and agar is the coagulant of the culture medium.
Formulation
| Ingredients | /liter |
| Enzymatic digest of casein | 17.0g |
| Enzymatic digest of animal tissue | 3.0g |
| Sorbitol | 10.0g |
| Bile salt No.3 | 1.5g |
| Sodium chloride | 5.0g |
| Neutral red | 0.03g |
| Crystal violet | 0.001g |
| Agar | 15.0g |
| pH 7.1±0.2 at 25°C | |
Preparation
Weigh 51.5g of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, dispense into Erlenmeyer flasks, and sterilize at 121℃ for 15min. Melt the sterilized culture medium and cool it to about 45℃, add 1 tube of supporting reagent (SR0240) (0.25mg potassium tellurite and 0.005mg Cefixime) to every 100mL of basal culture medium, mix and pour into the plate.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 18-24hours
| Quality control strains | Growth | Colony color |
| Escherichia coli ATCC25922 | PR≥0.7 | pale pink to red |
| Escherichia coli 0157:H7 NCTC12900 | PR≥0.7 | colorless |
| Staphylococcus aureus ATCC 25923 | Total inhibition | – |
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials Principle and Interpretation Enzymatic ……
The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300 bp to 5000 bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Figure 1 / 1
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MidRanger 1kb DNA Ladder (Cat# 11700) – 100 loads
Ladder Properties:
• Ten discrete fragments ranging from 300 bp to 5000 bp
| Fragment | Size (bp) | Mass (ng) |
| 1 | 5000 | 88 |
| 2 | 4000 | 78 |
| 3 | 3000 | 67 |
| 4 | 2500 | 58 |
| 5 | 2000 | 50 |
| 6 | 1500 | 43 |
| 7 | 1000 | 25 |
| 8 | 700 | 34 |
| 9 | 500 | 33 |
| 10 | 300 | 24 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
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