endo-BCN-PEG3-Boc-amine is a click chemistry linker containing a Boc-protected amine. The protected amine can be deprotected under mild acidic conditions. The BCN group enable copper free click chemistry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG3-Boc-amine is a click chemistry linker containing a Boc-protected amine. The protected amine can be deprotected under mild acidic conditions. The BCN group enable copper free click chemistry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Norgen LowRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our LowRanger contains eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for fast running times and accurate visual determination.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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LowRanger 100bp DNA Ladder (Cat# 11500) – 100 loads
Ladder Properties:
• Eleven discrete bands, ranging from 100 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference.
| Fragment | Size (bp) | Mass (ng) |
| 1 | 2000 | 110 |
| 2 | 1500 | 83 |
| 3 | 1000 | 55 |
| 4 | 800 | 44 |
| 5 | 700 | 39 |
| 6 | 600 | 33 |
| 7 | 500 | 55 |
| 8 | 400 | 22 |
| 9 | 300 | 17 |
| 10 | 200 | 22 |
| 11 | 100 | 22 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Usages:
For enumerating and enriching nonfastidious or fastidious bacteria.
Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance.
Formulation(per liter):
Pancreatic Digest of Casein 15g
Papaic Digest of Soybean 5g
Sodium chloride 5g
Agar 15g
Final pH 7.3±0.2
How to use:
1.Suspend 40g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
| Clone | IHC053 |
| Source | Mouse Monoclonal |
| Positive Control | Colon Carcinoma |
| Dilution Range | 1:200 |
p53, also known as tumor protein 53 or TP53, is a tumor suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumors, including those of the breast, ovarian, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.
