DBCO-S-S-acid is a cleavable reagent for introduction of a carboxylic acid moiety to azide-containing biomolecules using copper-free Click Chemistry. PEG spacer arm provides better solubility to the labeled molecules in aqueous media. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.
DBCO-S-S-acid is a cleavable reagent for introduction of a carboxylic acid moiety to azide-containing biomolecules using copper-free Click Chemistry. PEG spacer arm provides better solubility to the labeled molecules in aqueous media. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.
Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.
Vibrio cholerae TaqMan PCR Kit, 100 reactions
Vibrio cholerae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM38550 (100 preps) | Cat. TM38510 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| V. cholerae Primer & Probe Mix | 280 μL | 280 μL |
| V. cholerae Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Different sizes:
Powerful magnets quickly secure beads.
Simply turn tube for efficient bead mixing.
| Clone | IHC409 |
| Source | Mouse Monoclonal |
| Positive Control | Colon, Colon Carcinoma |
| Dilution Range | 1:200 |
MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumors that are microsatellite-unstable.
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