DBCO-S-S-acid is a cleavable reagent for introduction of a carboxylic acid moiety to azide-containing biomolecules using copper-free Click Chemistry. PEG spacer arm provides better solubility to the labeled molecules in aqueous media. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.
DBCO-S-S-acid is a cleavable reagent for introduction of a carboxylic acid moiety to azide-containing biomolecules using copper-free Click Chemistry. PEG spacer arm provides better solubility to the labeled molecules in aqueous media. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.
This product supplies a simple and rapid extraction of total RNA including microRNA from Blood, buffy coat, bone marrow, Cell suspension and other body fluids. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on.
The Kit can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤ 1 x106 cells suspension, 50 μl Whole Blood, 50μl buffy coat, 20μl bone marrow. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. RNA/DNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and
Kit Contents
| Contents | R661101C | R661102C | R661103C |
| Purification times | 48 Preps | 96 Preps | 480 preps |
| MagPure RNA Particles | 1.0 ml | 1.5 ml | 6 ml |
| Proteinase K | 24 mg | 48 mg | 220 mg |
| Protease Dissolve Buffer | 1.8 ml | 1.8 ml | 15 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 µl |
| DNase Buffer | 15 ml | 15 ml | 60 ml |
| Buffer AL | 10 ml | 10 ml | 60 ml |
| Buffer MCB* | 9 ml | 9 ml | 30 ml |
| Buffer MW1* | 13 ml | 22 ml | 110 ml |
| Buffer MW2* | 6 ml | 20 ml | 100 ml |
| RNase Free Water | 10 ml | 15 ml | 120 ml |
Storage and Stability
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.
This product supplies a simple and rapid extraction of total RNA including microRNA from Blood, buffy coat, bone marrow, Cell suspension and other body fluids. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on.
Propargyl-PEG2-t-butyl ester is a PEG linker that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper will be required for catalyzation. Under acidic conditions, the t-butyl group can be removed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG2-t-butyl ester is a PEG linker that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper will be required for catalyzation. Under acidic conditions, the t-butyl group can be removed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
QPCR is the best method for library quantification. Our reagent only amplifies library molecules that will be used for subsequent emPCR, and is optimized for amplification of various samples. Our reagent is compatible with commercial SYBR Green based QPCR reagents. Quantification of library concentration is achieved by comparison with a standard curve generated from DNA Standards.
The kit comprises DNA Standards (six 10-fold dilutions) and a primer mix.
NGS Library Quantification Standards with PCR Primers (Ion Torrent platform): real time quantitative PCR curve of the standards.
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
