Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).

Advantages

• Fast – column purification without PK digestion
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG

Kit Contents

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Overview

• Free of all DNases, RNases and nucleic acids
• Prepared without the use of DEPC
• Room temperature storage

Norgen’s Nuclease-Free Water is free of all DNases, RNases and nucleic acids and is suitable for all molecular biology applications requiring nuclease-free water including PCR, RT-PCR and real-time PCR.  Our water is prepared without the use of DEPC, and is ready to use without further treatment.

Available Sizes

• 4 x 1.25 mL
• 100 x 1.25 mL
• 100 mL
• 500 mL
• 1000 mL

Details

Applications
For use in any molecular biology application

Storage Conditions
This kit is stable for 2 years after the date of shipment.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.

Introduction

Usage: For isolating lactose-fermenting Gram-negative enteric bacilli.

Approximative Formula(Per Liter)

Pancreatic Digest of Gelatin                    17g

Peptones(meat and casein)                      3.0g

 Lactose Monohydrate                             10g 

Sodium Chloride                                      5g

 Bile Salts                                               1.5g

Agar                                                       13.5g

 Neutral Red                                          30mg 

Crystal Violet                                        1mg

Final pH 7.1±0.2  

Directions: Suspend 50g in 1 L of distilled or deionized water. Heat with frequent agitation to dissolve. Boil 1~2min. Distribute into flasks. Cool to 45~50°C and pour into sterile petri dishes. 

Storage: Keep container tightly closed, store in a dry place, away from bright light.

500g