Portable 4000rpm TD4C Centrifuge Machine for Lab, PRP & PRF

Application: 

Widely used in the laboratory, hospital, clinic and so on.

Product Features:

1. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.

2. Rang of speed from 0~4000rpm, smooth in operation, low noise and small vibration.

3. Micro-computer control system, digital display the RCF, time and speed.

4. Electric cover lock, compact design, super speed and imblance protection.

5. Small and portable, very convenient to use.

Technical Parameters:

Max.   Speed	4000rpm		Speed Accuracy	±20rpm
Max.Volume	8x20ml		Acc/Dec	10 Kinds
Max. RCF	1880xg		Power Supply	AC110V/220V 50HZ/60HZ
Timer	0~99min		Noise	≤55dBA
Dimension	310x270x220mm		Net Weight	6.5Kg
Warranty	1 Year		Certifications	CE, ISO &   Calibration report are available.

Matched Rotors:

Order   No.	Rotor Type	Max.Speed(rpm)	Max.Volume(ml)	Max.RCF(xg)
4C-1	Angle Rotor	4000	8x15ml/20ml	1800/1840
4C-2	Angle Rotor	4000	6x10ml/15ml/20ml	1790
4C-3	Angle Rotor	4000	12x5ml	1540
4C-4	Angle Rotor	4000	12x10ml/7ml	1792
4C-5	Angle Rotor	4000	8x10ml vacuum tube	1790
			8x5ml vacuum tube adapter	1790
			8x7ml vacuum tube adapter	1790

Introduction

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 10⁷) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from yeast cultures
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Yeast culture
Sample amount	Bacterial culture: 1-1.5 ml
Elution volume	≥30μl
Time per run	≤60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).

Advantages

• Fast – several samples can be extracted in 40 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• Good repeatability – silica technology can obtain ideal results every time
• High recovery – DNA can be recovered at the level of PG
• Sufficient components – lysozyme, protease K, RNase A and glass beads

Kit Contents

Contents	D314702	D314703
Purification Times	50 Preps	250 Preps
Hipure DNA Mini Columns I	50	250
2ml Collection Tubes	50	250
Glass Beads (0.4~0.6mm)	20 g	90 g
Buffer SE	30 ml	150 ml
Lyticase	1.8 ml	5 x 1.8 ml
Buffer ATL	30 ml	150 ml
ReagentDX	500 μl	1500 μl
Buffer DL	30 ml	150 ml
Buffer GW1*	13 ml	66 ml
Buffer GW2*	20 ml	2 x 50 ml
Proteinase K	12 mg	60 mg
Protease Dissolve Buffer	1.8 ml	5 ml
Buffer AE	15 ml	30 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (

Overview

• Detection kits for WNV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.

WNV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM44250 (100 preps)	Cat. TM44210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
WNV Primer & Probe Mix	280 μL	280 μL
WNV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1