DBCO-NHCO-PEG4-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG4-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue, cell |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Tissues, cells, lymphocytes and other clinical sample |
| Sample amount | Cells grown in suspension:3~5 x 106Animal tissue: 10~20mgPlant tissue: ≤100mg |
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water
Advantages
Kit Contents
| Contents | IVD3020 |
| Purification Times | 200 Preps |
| MagPure RNA Particles | 7 ml |
| DNase I | 4 x 600 µl |
| DNase Buffer | 80 ml |
| RTL Lysis Buffer | 150 ml |
| Buffer MCB* | 75 ml |
| Buffer MW1* | 110 ml |
| Buffer MW2* | 50 ml |
| RNase Free Water | 60 ml |
| Cat.No | Reagent | IVD3020-F-96 |
| DNase Buffer | 60 ml | |
| DNase I | 2 x 600 μl | |
| RTL Lysis Buffer | 80 ml | |
| Buffer MCB | 18 ml | |
| 96-Tip | 1 | |
| Sample plate (DW Plate) | 500μl Buffer MCB | 1 |
| Wash 1 Plate (DW Plate) | 700μl Buffer MW130μl MagPure RNA Partilces | 1 |
| DNase Plate | Empty | |
| Wash 2 Plate (DW Plate) | 700μl Buffer MW1 | 1 |
| Wash 3 Plate (DW Plate) | 900μl Buffer MW2 | 1 |
| Elution plate (DW Plate) | 80μl RNase Free Water | 1 |
| Cat.No | Reagent | IVD3020-TL-06 |
| Purification times | 96 Preps | |
| DNase I | 2 x 600 μl | |
| DNase Buffer | 60 ml | |
| RTL Lysis Buffer | 60 ml | |
| Buffer MCB | 40 ml | |
| 96-Tip | 12 PCS | |
| 2.0ml V-bottom plate | Row 1/7：500μl Buffer MCBRow 2/8：500μl Buffer MW1Row 3/9：emptyRow 4/10：30μl Magpure RNA Particles500μl Buffer MW2 Row 5/11：900μl Buffer MW2 Row 6/12：80μl RNase Free Water | 6 |
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is the etiological agent of the sexually transmitted infection (STI) gonorrhoea, which globally causes an estimated 60 million new cases of gonococcal disease annually. It is second only to Chlamydia trachomatis as the most reported notifiable sexually transmitted disease. Infections with N. gonorrhoeae are primarily restricted to the mucus membranes of the endocervix, urethra, rectum, and pharynx. In females, gonorrhoea is a major cause of pelvic inflammatory disease and may lead to tubal infertility, ectopic pregnancy, and chronic pelvic pain, whereas in males it primarily causes urethritis. Importantly, these infections may often be asymptomatic, thereby contributing to further transmission and maintenance of the disease within populations.
Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard is prepared from pelleted bacteria grown on culture plates using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Neisseria gonorrhoeae.
Figure 1 / 1
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Volume Provided – 250 µL
DNA Quantity – 2 x 104 copies per µL
Storage Conditions
Upon receipt, store Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20oC or lower.
These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.
Endotoxin Removal Kit (Mini)
This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.
Endotoxin Removal Kit (Midi)
This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
Endotoxin Removal Kit (Maxi)
This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
About Endotoxins
Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
Figure 1 / 2
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| Kit Specifications | |
| Maximum DNA Input | 25 μg |
| Final Endotoxin Level | ≤ 0.1 EU/µg DNA |
| Size of DNA Purified | Up to 13,000 bp |
| Maximum DNA Volume Input | 100 μL |
| Average Recovery | > 90% |
| Time to Complete 10 Purifications | 20 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 22700 (25 preps) | Cat. 52200 (10 preps) | Cat. 21900 (4 preps) |
|---|---|---|---|
| Buffer SK | 15 mL | 60 mL | 60 mL |
| Wash Solution H | 18 mL | 18 mL | 18 mL |
| Elution Buffer I | 6 mL | 12 mL | 12 mL |
| Endotoxin Removal Solution | 1.5 mL | 1.5 mL | 1.5 mL |
| Precipitation Solution | – | 1.5 mL | 1.5 mL |
| Spin Columns (assembled with Collection Tubes) | – | 10 | 4 |
| Spin Columns | 25 | – | – |
| Collection Tubes | 25 | – | – |
| Elution Tubes (1.7 mL) | 25 | – | – |
| Elution Tubes (15 mL) | – | 10 | – |
| Elution Tubes (50 mL) | – | – | 4 |
| Product Insert | 1 | 1 | 1 |
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Tel : 081-875-1869 , 02-328-7179
Email : [email protected]
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