N-(Amino-PEG4)-N-bis(PEG4-propargyl) HCl salt is a trifunctional linker with two terminal alkynes and a primary amine. The terminal alkynes can be linked to azides using copper click chemistry while the primary amine is reactive towards carboxylic acids, NHS esters, ketones and aldehydes to form a variety of stable bonds.
N-(Amino-PEG4)-N-bis(PEG4-propargyl) HCl salt is a trifunctional linker with two terminal alkynes and a primary amine. The terminal alkynes can be linked to azides using copper click chemistry while the primary amine is reactive towards carboxylic acids, NHS esters, ketones and aldehydes to form a variety of stable bonds.
This kit provides a rapid spin column procedure for the isolation of exosomal RNA from urine samples. Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays such as RT-PCR, qRT-PCR, NGS, microarrays and more. The protocol can be completed in under 50 minutes. Urine volumes of 1 to 10 mL can be processed easily and rapidly. All sizes of RNA are recovered at an equal rate without the need for using hazardous chemicals like phenol.
Background
Exosomes are 40 – 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. These vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which can inform about the cell type from which the exosomes are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNases they can be efficiently recovered from biological fluids, such as urine.
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| Kit Specifications | |
| Minimum Urine Input | 1 mL |
| Maximum Urine Input | 10 mL |
| Size of RNA Purified | Small exosomal RNA species |
| Time to Complete Purification | ~ 50 minutes |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 47200 (50 preps) |
|---|---|
| Slurry B1 | 18 mL |
| Binding Buffer A | 20 mL |
| Lysis Buffer A | 2 x 20 mL |
| Wash Solution A | 38 mL |
| Elution Solution A | 6 mL |
| Mini Filter Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
DBCO-PEG2-DBCO is a PEG linker containing two terminal DBCO groups. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. T Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG2-DBCO is a PEG linker containing two terminal DBCO groups. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. T Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
