Tetra(3-methoxy-N-(prop-2-ynyl)propanamide) Methane is a 4-branched molecule with propargyl groups that can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit on the amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.

Details

Principles

SolPure DNA Kits is an improved salt precipitation purification method. (Blood samples are lysed in red blood cell lysis buffer to remove red blood cells, and white blood cells are collected by centrifugation.) After lysis, DNA is released into the lysis buffer. RNA is removed by RNASE A. High salt solution is added to precipitate proteins and impurities. Centrifuge to remove precipitate and obtain supernatant containing only DNA. Add isopropanol to precipitate and recover DNA. Wash with 70% ethanol to remove salt, and finally add Buffer TE to dissolve DNA.

Kit Contents

Contents	D3317-01	D3317-02	D3317-03
Purification Times	10	50	250
10 x Buffer RBC	4 ml	50 ml	100 ml
Buffer STE	60 ml	250 ml	2 x 550 ml
Buffer SDS (20%)	6 ml	25 ml	100 ml
Buffer PPS	20 ml	90 ml	400 ml
Proteinase K	12 mg	50 mg	240 mg
Protease Dissolve Buffer	1.8 ml	10 ml	20 ml
RNase A	5 mg	20 mg	60 mg
Buffer TE	10 ml	60 ml	250 ml

Storage and Stability

RNase A and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Nineteen discrete fragments ranging from 100 bp to 10000 bp
• Higher intensity reference bands at 500 bp, 1000 bp and 5000 bp

The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100 bp to 10000 bp with higher intensity reference bands at 500 bp, 1000 bp and 5000 bp. This Ladder is ideal for size determination of digested DNA.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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HighRanger Plus 100 bp DNA Ladder (Cat# 12000) – 100 loads

Ladder Properties:

• Nineteen discrete fragments ranging from 100 bp to 10000 bp
• Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	10000	42
2	8000	33
3	6000	25
4	5000	42
5	4000	35
6	3000	27
7	2500	22
8	2000	18
9	1500	13
10	1000	40
11	900	28
12	800	25
13	700	34
14	600	19
15	500	44
16	400	13
17	300	18
18	200	13
19	100	10

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
This kit is stable for 2 years after the date of shipment.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.