DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Virgin Polypropylene 96 Square Well plate with high uniformity from well to well.
Product Details
Application:
DNA Nucleic Acid
Format:
NFO Kit
Kit Components:
Reagents,Buffer,Enzymes
Manufacturer:
Amp-future Bio
Product Name:
DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)
Reaction Time:
5-20mins
Reaction Volume:
50μL
Sensitivity:
500-1000copies/ml
Shelf Life:
14 Months
Specificity:
High
Storage Conditions:
-20℃
Suitability:
Universal
High Light:
,
,
Payment & Shipping Terms
Minimum Order Quantity
48T
Price
USD$3.8/T
Packaging Details
16T/Bag,48T/Box
Delivery Time
6 working days
Payment Terms
T/T, MoneyGram
Supply Ability
100000T/Month
Product Description
【Principle Overview】
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
【Primer design】
It is recommended to use primers with a length of 30-35 bp.Too short primers will affect the amplification speed and detection sensitivity;the 5′ end of the reverse primer is labeled with a modification group (commonly used biotin).
Primers are designed to avoid the formation of secondary structures that affect amplification; the length of the amplicon is recommended to be 150-500bp.
【Colloidal gold probe design】
In the middle of the forward and reverse primer,design a sequence of 46-52nt in length that is complementary to the target fragment; modify an antigen marker (typical FAM) at the 5′ end;mark a dSpacer (tetrahydrofuran, THF) in the middle of the 5′ end and the 3′ end ), as the recognition site of nfo;the 3′ end is labeled with a modification group, such as amine group, phosphate group or C3-Spacer, etc.
【Features】
This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 15 minutes),and the reaction components are in dry powder state,which is easy to operate and easy to store.
This reagent has low equipment requirement,and the reaction operation can be carried out in metal baths, water baths, etc.,and there is no need to purchase expensive exclusive equipment such as PCR amplifiers.
| Composition | Content |
| A buffer | 1.6mL×1Tube |
| B buffer | 150μL×1Tube |
| Positive control template-II | 100μL×1Tube |
| Positive control probe and primer mix-II | 70μL×1Tube |
| Reagent | 48 T |
| Guide Manual | 1 Copy |
【Kit storage】
1. Storage conditions: storage temperature ≤ -20℃constant temperature environment, keep away from light and avoid heavy pressure;
2. Product validity period: 14 months;
3. See the outer packaging for the production date.
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
The Permagen 96-Well Side Pull Bar Magnet PCR Separation Plate was designed for use in manual or automation applications where pulling beads to one side of the wells is essential. Also available in a kit form which includes our MTP96 clear top plate for 0.2 mL PCR tubes and strips.
Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added two angled, partial frames around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets when using an automated liquid handling robot.
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
MSP750 (Magnet Plate only)
MSP750KT (Includes top plate kit)
Minimum – 30 µL
Maximum – 0.2 mL
The Permagen 96-Well Side Pull Bar Magnet PCR Separation Plate was designed for use in manual or automation applications where pulling beads to one side of the wells is essential. Also available in a kit form which includes our MTP96 clear top plate for 0.2 mL PCR tubes and strips.
