DBCO-C3-alcohol

Name of Product

Microsporidia – IFT

Catalog Number

AF 8100

Short Info

Indirect immunofluorescence assay for the specific diagnostic of human intestinal microsporidiosis.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

Indirect immunofluorescence test

Range/Assay Sensivity

Test Principle

Monoclonal antibodies bind specifically to samples with spores (E. bienusi and E. intestinalis) attached to the slide wells. Unfixed antibodies will be washed away. The presence of spore-specific antibodies is detected with a fluorescent anti-mouse IgG conjugate.

2 x 50 tests
Indirect immunofluorescence assay for the specific diagnostic of human intestinal microsporidiosis.

Overview

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification

Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl₂, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Details

Supporting Data

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Reagents Supplied – Cat # 28007

•  2X PCR Master Mix (1 Vial, 100 Reactions) – Sufficient reagent for 100 x 20 µL reactions

Storage Conditions and Product Stability
Norgen’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Kit contents

1. Champion™ Competent Cells
2. pUC19 Control Plasmid (5 μl, 10^-4 μg/μl)
3. Champion™ Transformation Protocol Card

Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C. 

Storage and expiration 

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.