DBCO-PEG2-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG2-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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| Kit Specifications | |
| Maximum Binding Capacity Per Column | Up to 50 μg RNA |
| Maximum Loading Volume Per Spin Column | 650 μL |
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications | 25 minutes |
| Maximum Amount of Starting Material | |
| Liver Tissue | 20 mg |
| Heart Tissue | 5 mg |
| Kidney Tissue | 20 mg |
| Brain Tissue | 25 mg |
| Lung Tissue | 20 mg |
| Spleen Tissue | 20 mg |
| Whole Blood | 100 µL |
| Hela Cells | 3 x 106 |
| CHO | 3 x 106 |
| Yeast | 1 x 108 cells |
| E.coli | 1 x 109 CFU/mL |
| Bacteria | 1 x 109 cells |
| Plasma/Serum | 200 µL |
| Fungi | 50 mg |
| Plant Tissue | 50 mg |
| Viruses | ≤100 µL of viral suspension |
| Average Yield | |
| HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg | |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 48300 (50 preps) | Cat. 48400 (100 preps) |
|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL |
| RNA Purification Columns | 50 | 100 |
| Genomic DNA Removal Columns | 50 | 100 |
| Collection Tubes | 100 | 200 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free
Conforms to SBS and ANSI requirements
Centrifugal strength up to 3000g
Square well
50 count
Labware definition is available for immediate use in Opentron’s Labware Library
NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free
Conforms to SBS and ANSI requirements
Centrifugal strength up to 3000g
Square well
50 count
Labware definition is available for immediate use in Opentron’s Labware Library
R-AMHR4
SKU: 700005032
200 assays per kit (4 vials)
| Content: | 200 assays per kit (4 vials) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Limit of Detection: | 0.05 U/mL |
| Reproducibility (%): | ~ 3% |
| Reaction Time (min): | ~ 30 min |
High purity α-Amylase HR Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
A new amylase assay reagent containing p-nitrophenyl α-D-maltoheptaoside (blocked) plus a thermostable α-glucosidase. The incorporation of this enzyme allows assays to be performed at temperatures up to 60oC and over the pH range 5.2-7.5.
See our complete list of assay reagents.
High purity α-Amylase HR Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
A new amylase assay reagent containing p-nitrophenyl α-D-maltoheptaoside (blocked) plus a thermostable α-glucosidase. The incorporation of this enzyme allows assays to be performed at temperatures up to 60oC and over the pH range 5.2-7.5.
See our complete list of assay reagents.
