DBCO-PEG4-Maleimide is PEG linker containing a maleimide group and a DBCO moiety. The hydrophilic PEG spacer arm improves solubility in aqueous buffers. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Features:
1.Brushless frequency motor,in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling,cold and hot alternating easily, free environment pollution and precise in temperature control.
3. Microprocessor control, digital display which indicates the speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
6. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
7. There are several kinds of rotors for your choice, adapters can be designed by experimental requirements.
8. 3 tiers protection steel cover and get the ideal centrifugation result.
TDL5M Technical Parameter：
| Max. Speed | 5000rpm |
| Max. RCF | 4730×g |
| Max. Capacity | 4×800ml( 4*450ml blood bags) |
| Time Range | 0~99h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 65 |
| Temperature | -20℃-40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 700×715×420mm |
| Net Weight(Kg) | 125KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TDL5M
| Order No. | Rotor | Max Speed (rpm) | Max Volume(ml) | Max. RCF（×g） |
| NO30671 | Swing Rotor | 4000 | 4×800ml(Round Cup) | 3450 |
| NO30696 | Swing Rotor | 4000 | 4×500ml( Round Cup) | 3380 |
| NO30679 | Swing Rotor | 4000 | 4×500ml(Square Cup) | 3310 |
| NO31494 | Microplate Rotor | 4000 | 4×4×96 Well | 2840 |
| NO31491 | 4000 | 2×4×96 Well | 2490 | |
| NO31376 | 4000 | 2×3×48 Well | 2300 | |
| NO30690 | Swing Rotor | 4000 | 4×30×7ml | 3270 |
| 4×30×5ml | 2890 | |||
| 4×18×10ml | 3140 | |||
| NO30607 | Angle Rotor | 5000 | 12×15ml | 3080 |
| NO30611 | Angle Rotor | 5000 | 6×50ml | 2850 |
| NO30640 | Angle Rotor | 5000 | 4×50ml | 2520 |
| NO30613 | Angle Rotor | 5000 | 4×100ml | 2630 |
| NO30639 | Angle Rotor | 5000 | 24×15ml | 3500 |
| NO30641 | Angle Rotor | 5000 | 12×50ml | 3860 |
| NO30614 | Angle Rotor | 5000 | 6×100ml | 3130 |
| NO30642 | Angle Rotor | 4000 | 12×100ml | 2970 |
Adapter for 800ml and 500ml bucket
 VolumeCup | 5ml | 7ml | 10ml | 15ml | 50ml |
| 800ml（Round Cup） | 23pcs/cup | 23 | 19 | 14 | 5 |
| 500ml（Round Cup） | 18pcs/cup | 18 | 14 | 9 | 3 |
| 500ml（Square Cup） | 20pcs/cup | 20 | 16 | 12 | 5 |
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
DBCO-PEG10-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG10-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.