m-PEG24-DBCO

Overview

• Fast, reproducible and easy processing of samples
• High yield and high quality genomic DNA with no RNA or protein contamination
• DNA ready for any application including PCR, qPCR, genotyping and more

Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.

Cells and Tissue DNA Isolation Kit (Spin Column)

Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Micro Kit (Micro)

Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.

Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 9

Previous

Next

Click for expanded view

Kit Specifications
Maximum Cells and Tissue Input	20 mg of animal tissue 3 x 106 cells
Average Yield*	8-10 µg (20 mg of animal tissue) 8-12 µg (3 x 106 cells)
Average Purity (OD260/280)	1.8 – 1.9
Time to Complete 10 Purifications	40 minutes hands-on time (Cat. 59100) 50 minutes hands on time (Cat. 62500)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Component	Cat. 53100 (50 preps)	Cat. 57300 (50 preps)	Cat. 59100 (50 preps)	Cat. 62500 (192 preps)
Lysis Buffer B	20 mL	20 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	18 mL	18 mL	55 mL
Wash Solution A	18 mL	18 mL	–	–
Elution Buffer B	30 mL	8 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K	1.2 mL	1.2 mL	1.2 mL	–
Proteinase K in Sotrage Buffer	–	–	–	4 mL
Magnetic Bead Suspension	–	–	2 x 1.1 mL	8.5 mL
96-Well Plate	–	–	–	2
Spin Columns	50	–	–	–
Micro Spin Columns	–	50	–	–
Collection Tubes	50	50	–	–
Elution Tubes (1.7 mL)	50	50	50	–
96-Well Elution Plate	–	–	–	2
Adhesive Tape	–	–	–	2
Product Insert	1	1	1	1

Sulfo DBCO-Maleimide a water-soluble sulfhydryl reactive containing a maleimide group and a DBCO moiety. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Sulfo DBCO-Maleimide a water-soluble sulfhydryl reactive containing a maleimide group and a DBCO moiety. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DL6MB Technical Parameter：

Max. Speed	6000rpm
Max. RCF	6600×g
Max. Capacity	6×1000ml
Timer	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature Range	-20~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V  50HZ/60HZ
Size (L x W x Hmm)	800×740×930mm
Net Weight(Kg)	310KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for DL6MB

Order No.	Rotor	Max Speed (rpm)	Max Volume(ml)	Max.RCF（×g）
6MB-1	Swing Rotor	4200	6×1000ml	5100
6MB-2	Angle Rotor	6000	4×300ml	5390
6MB-3	Angle Rotor	6000	6×300ml	5660
6MB-4	Angle Rotor	6000	6×500ml	6600

Features

1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.

2. Frequency conversion motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. With pre-cooling function,Digital display which indicates the program, speed, time,RCF, temperature.

4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.

5. There are several kinds of rotors for your choice.

6. Electric lid lock, compact design, super speed and imbalance protection.

7. The centrifuge body is made of high-quality steel, safe and reliable.