N-(Amino-PEG4)-N-bis(PEG4-propargyl) HCl salt is a trifunctional linker with two terminal alkynes and a primary amine. The terminal alkynes can be linked to azides using copper click chemistry while the primary amine is reactive towards carboxylic acids, NHS esters, ketones and aldehydes to form a variety of stable bonds.
Detail
N-(Amino-PEG4)-N-bis(PEG4-propargyl) HCl salt is a trifunctional linker with two terminal alkynes and a primary amine. The terminal alkynes can be linked to azides using copper click chemistry while the primary amine is reactive towards carboxylic acids, NHS esters, ketones and aldehydes to form a variety of stable bonds.
The Opentrons Deep Well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
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The Opentrons Deep Well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
D6311-F-96 MagPure Blood DNA Precast Kit (96 channel machine)
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 200μl whole blood
Applications
PCR, southern bolt and virus detection, etc
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount
200μl
Elution volume
≥50μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
Fast – polydisperse magnetic beads, fast magnetic response and short extraction time
High purity – the obtained DNA can be directly used for second-generation sequencing, PCR based detection, gene bank, etc.
Automatic – saving time andlabor and safer
Kit Contents
Contents
D631101
D631102
D631103
Purification Times
48
96
480
MagPure Particles
1.2 ml
2.5 ml
11 ml
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
1.8 ml
5 ml
15 ml
Buffer AL
15 ml
30 ml
120 ml
Buffer BD
5 ml
10 ml
50 ml
Buffer GW1
22 ml
53 ml
220 ml
Elution Buffer
15 ml
30 ml
100 ml
Storage and Stability
Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage(up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.