N-(Amine-PEG8)-N-bis(PEG8-Propargyl) is a trifunctional linker containing an amino group and two terminal alkynes. These alkynes are frequently used in copper (I) click reactions with azides to form stable triazole linkages, while the primary amine is reactive towards carboxylic acids and other carbonyl compounds such as NHS esters, aldehydes or ketones.
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N-(Amine-PEG8)-N-bis(PEG8-Propargyl) is a trifunctional linker containing an amino group and two terminal alkynes. These alkynes are frequently used in copper (I) click reactions with azides to form stable triazole linkages, while the primary amine is reactive towards carboxylic acids and other carbonyl compounds such as NHS esters, aldehydes or ketones.
NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.
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NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Phytic Acid, Phosphorus
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
655
Signal Response:
Increase
Linear Range:
~ 0.5 to ~ 7.5 µg of phosphorus per assay
Limit of Detection:
~ 11.3 mg phosphorus (~ 40 mg phytic acid)
Reaction Time (min):
25 min enzymic; 1 h for phosphate determination
Application examples:
Seed materials, feeds and foodstuffs.
Method recognition:
Novel method
The Phytic Acid test kit is a simple method for the measurement and analysis of phytic acid/total phosphorus in food and feed samples. This method does not require purification of phytic acid via anion-exchange chromatography making it amenable to high numbers of samples.
Display our complete list of organic acid assay kits.
Advantages
Very cost effective
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
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The Phytic Acid test kit is a simple method for the measurement and analysis of phytic acid/total phosphorus in food and feed samples. This method does not require purification of phytic acid via anion-exchange chromatography making it amenable to high numbers of samples.