Low Abundance DNA Quantification Kit

Detail

Overview

Quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range
Compatible with any Real-Time PCR system
DNA is accurately quantified using a standard curve constructed from the provided DNA standard

Norgen’s Low Abundance DNA Quantification Kit offers a PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range. The kit consists of a specially designed primer mix, that is used in conjunction with the provided 2x PCR Master Mix, to amplify human DNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown DNA is accurately quantified by using a standard curve constructed from the provided DNA Standard.

Details

Supporting Data

Figure 1 / 2

Figure 1. Sensitivity of Norgen's Kit Compared to Other Methods

Figure 1. Sensitivity of Norgen's Kit Compared to Other Methods

Figure 2. Sensitivity of DNA Quantification in the Picogram Range

Click for expanded view

Storage Conditions
Upon receipt, store Norgen’s Low Abundance DNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycle. If needed, prepare smaller working aliquots and store at -20°C or lower.

Component	Cat. 57200 (48 reactions)
2X PCR Master Mix	1 mL
DNA Quantification Primer Set Mix	200 µL
Quantified DNA Standard	5 standards, each 100 µL
Nuclease-Free Water	1.25 mL
Product Insert	1

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Saliva RNA Collection and Preservation Devices

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Product Info

Overview

Reliable and cost-effective
Non-invasive, user-friendly sample collection and preservation in one convenient kit
Preserved RNA is stable for up to 2 months at room temperature
Compatible with most RNA isolation methods including Norgen’s Saliva/Swab RNA Purification Kits
High quality RNA is suitable for sensitive downstream applications including RT-PCR, RT-qPCR, RNA sequencing and microarray
Inactivates viruses including SARS-CoV-2 upon mixing – Explore the Application Note
Shipping accessories can be purchased separately
CE-IVD marked version available

Norgen’s Saliva RNA Collection and Preservation Devices are designed for simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices consists of 3 components:

Saliva Collection Funnel and Collection Tube
Collection Tube Cap
Norgen’s Saliva RNA Preservative contained within a sealed squeezable ampoule

Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The Saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for epidemiological and population studies.

Saliva RNA Preservative

Norgen’s Saliva RNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of RNA from fresh specimens. The buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for up to 2 months at room temperature.

Nucleic Acid Isolation from Preservative

Saliva RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300).

Details

Supporting Data

Figure 1 / 4

Figure 1. Stability of RNA at room temperature for up to 2 months

Figure 1. Stability of RNA at room temperature for up to 2 months

Figure 2. Stability of three house keeping genes over a 2 month period

Figure 3. Norgen’s Saliva RNA Preservative is able to successfully preserve saliva RNA samples

Figure 4. Detection of Viral, Bacterial and Human RNA from Preserved Saliva Samples.

Click for expanded view

Kit Specifications
Volume of Saliva Collected	2 mL
Volume of Saliva-Preservative Mix	4 mL
Preservation Temperature	Room Temperature
Preservation time	Up to 2 months at room temperature

Shelf Life and Handling

When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
Once collected, saliva RNA is stable for up to 2 months when kept tightly sealed and stored at room temperature.
The Collection Tube, the Collection Funnel and the Device Container of each individual Saliva RNA Collection and Preservation Device are recyclable.

Kit Components	Cat. RU53800 (50 Devices)
Individual Saliva RNA Collection and Preservation Devices	50
Donor Procedure Flowchart	1
Product Insert	1

Individual Saliva RNA Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube	1
Collection Tube Cap	1
Preservative Ampoule	1
Donor Instructions	1

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