Overview
- Quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range
- Compatible with any Real-Time PCR system
- RNA is accurately quantified using a standard curve constructed from the provided RNA standard
Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard.
Details
Supporting Data
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Storage Conditions
Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
| Component | Cat. 58900 (48 reactions) |
|---|---|
| microScript microRNA Enzyme Mix | 20 μL |
| 2x microScript Reverse Transcriptase Reaction Mix | 0.5 mL |
| 2X Real-Time PCR Master Mix | 1 mL |
| RNA Quantification Primer Set Mix | 200 μL |
| Quantified RNA Standard 50 pg/μL | 2 x 60 μL |
| Nuclease-Free Water | 3 x 1.25 mL |
| Product Insert | 1 |
