Overview
- Robust Lysis Solution processes even the most challenging plant species such as pine needle and grape
- No phenol extractions
- DNA and all sizes of RNA are recovered, including microRNA
- High quality DNA and RNA are purified simultaneously using the same spin column
- No need to split the lysate
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides for rapid spin column isolation and purification of total RNA and genomic DNA simultaneously from a single plant sample without splitting the lysate. Norgen’s plant lysis solution is highly robust and effective over a wide range of plants including challenging samples. The total RNA and genomic DNA are both column purified in under 30 minutes. Since RNA and DNA are isolated without splitting the lysate, variability and inconsistent results are reduced. All sizes of RNA including microRNA are recovered without the need for phenol. Optional on-column DNase and RNase treatments provide flexibility to isolate DNA-free RNA or RNA-free DNA respectively. Isolated nucleic acids are of a high quality and yield, and are ready for downstream use including PCR, qPCR, RT-PCR, qRT-PCR, sequencing and more.
Background
It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. This is of great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.
Details
Supporting Data
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| Kit Specifications | |
| Column Binding Capacity | 50 μg for RNA 15 μg for genomic DNA |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells | 100 mg 5 x 106 |
| Time to Complete 10 Purifications | 30 minutes |
| Average Yields* Peach Leaves (100 mg) | 40 μg RNA, 5 μg gDNA |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. 24400 (50 preps) |
|---|---|
| Lysis Buffer M | 40 mL |
| Binding Buffer I | 7 mL |
| Solution WN | 18 mL |
| Wash Solution A | 38 mL |
| Elution Buffer E | 20 mL |
| Enzyme Incubation Buffer B | 6 mL |
| Filter Columns | 50 |
| Spin Columns | 50 |
| Collection Tubes | 100 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
