Hepatitis B virus (HBV) TaqMan PCR Detection Kits

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Detail

Overview

  • Detection kits for the HBV
  • Available in TaqMan format for analysis

The Hepatitis B virus (HBV) is mainly transmitted via blood or blood products. In addition, sexual, oral and perinatal infections are also possible. Early symptoms of the infection include appetite loss, vomiting and abdominal symptoms, with approximately 10-20% of those patients developing fever as well as rheumatoid joint and muscle pain. Jaundice, which may be accompanied by itching, will then develop within 2-14 days. Fulminant hepatitis then occurs in about 1% of all infected patients, which in severe cases may be fatal. Of those individuals infected by HBV, 5-10% will develop chronic liver inflammation which may progress to cirrhosis of the liver or, in the worst case, primary liver cell carcinoma. The HBV virus is a hepadnavirus that has a circular genome composed of partially double-stranded DNA.

HBV TaqMan PCR Kit, 100 reactions

  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents

HBV TaqMan PCR Probe/Primer Set and Controls, 100 reactions

  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Figure 1. HBV TaqMan PCR Kit
Figure 1. HBV TaqMan PCR Kit
Figure 2. HBV TaqMan PCR Kit
Figure 3. HBV TaqMan PCR Kit

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

ComponentCat. TM29250 (100 rxns)Cat. TM29210 (100 rxns)
MDx TaqMan 2X PCR Master Mix2 x 700 μL
HBV Primer & Probe Mix280 μL280 μL
HBV Positive Control150 μL150 μL
Nuclease-Free Water (Negative Control)1.25 mL1.25 mL
Product Insert11

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