Lyophilized format designed for room temperature shipping
Available in TaqMan format for analysis
Norgen’s EBV TaqMan PCR Lyophilized Kit is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s EBV TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
All kit components should be stored at -20°C upon arrival.
Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
Component
Cat. TM41050L (100 preps)
Cat. TM41010L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)
4 x 350 μL
–
EBV Primer & Probe Mix (Lyo)
280 μL
280 μL
EBV Positive Control (Lyo)
150 μL
150 μL
Nuclease-Free Water (Negative Control)
3 x 1.25 mL
1 x 1.25 mL
Product Insert
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Purple-Jelley Hyaluronic Acid assay kit
Product Info
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Product Info
Purple-Jelley Hyaluronic Acid assay kit
Biocolor’s Purple-Jelley assay kit is the perfect tool for accurate measurement of hyaluronic acid / Hyaluronan levels in your samples. This colorimetric assay is optimised for quantitative analysis in-vivo, tissue-derived hyaluronic acid / Hyaluronan and includes full step-by-step instructions.
Colorimetric Detection (655nm) (Endpoint)
Hyaluronic Acid: A gentle giant!
Hyaluronic acid, in its hydrated form, is a unique carbohydrate polymer, often referred to as a ‘gentle giant.’ It consists of a lengthy, flexible, non-branching chain with a repeating disaccharide pattern. This disaccharide is composed of alternating uronic acid and aminosugar units.
Why is our kit called ‘Purple-Jelley’?
Discovering the J-Aggregate Effect in Cyanine DyesIn 1936, Edwin Jelley made a fascinating observation, documented it in a letter to Nature (Nature 138, 1009 – 1010). He noted a peculiar behaviour of certain cyanine dyes, that when dissolved in 5 M NaCl, they dyes exhibited a third absorbance peak at a longer wavelength, around 650nm. In deionized water, however, they displayed only a double peak at approximately 540nm and 570nm. The 650nm peak in concentrated dye solutions resulted from the aggregation of dye molecules and was later termed a ‘J-aggregate,’ in honor of Edwin Jelley. The J-aggregate is known as a supra-molecular complex, formed by stacking individual dye molecules.
Subsequent research in the 1960s, notably by Kay et al. (J. Physical Chem. 68, 1896 – 1906), revealed that various biological polymers, including proteins, DNA, polar lipids, and glycosaminoglycans, could also induce this third absorbance peak. This phenomenon led to the development of the Purple-Jelley assay, named after the purple color of the dye reagent and Edwin Jelley himself.
An overview of the Purple-Jelley assay steps:
During the assay, hyaluronic acid is selectively purified during the assay sample preparation protocol. This is then reacted with the Purple-Jelley dye reagent, and the absorption of the characteristic third wavelength recorded. By comparison with a calibration curve the hyaluronic acid content of the sample can be measured.
Step 1. The assay protocol takes tissue samples through a sequential sample preparation protocol which involves enzymatic protein digestion, followed by precipitation and purification of GAGs, culminating in the precipitation of purified Hyaluronic acid.
Step2. The processed sample is then incubated for 10 minutes with the Purple-Jelley dye reagent, forming a coloured product which can be measured spectrophotometrically.
Step 3. The Hyaluronic acid content of unknown samples can be calculated by comparison against a calibration curve prepared using a standard comprising hyaluronic acid (supplied with the kit).
Assay range
10 – 100µg/ml
Limit of Detection
10µg/ml
Detection Method
Colorimetric Detection (655nm) (Endpoint)
Measurements per kit
100 in total (allows a maximum of 46 samples to be run in duplicate alongside a standard curve).
Suitable Samples
In-vivo: Hyaluronic acid purified from in-vivo tissues. The kit protocol involves extraction and purification of hyaluronic acid prior to reaction with the Purple-Dye reagent.
Precautions
This kit is designed for research use only. Not for use in diagnostic procedures. Kit requires access to a centrifuge, as well as a spectrophotometer/colorimeter capable of colorimetric, absorbance detection at 655nm. Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.
2. Hyaluronan Reference Standard (1x 5ml, 0.2mg/ml soluble Hyaluronic Acid)
3. Precipitating Reagent (2x 34ml)
4. Sodium Chloride (1x 20ml)
5. Cetylpyridinium Chloride (1x 20ml)
6. TRIS-buffered Saline (5x tablets)
7. 2ml screw-cap tubes for preparation of samples.
8. Assay kit manual
NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.
Document
Biocolor’s Purple-Jelley assay kit is the perfect tool for accurate measurement of hyaluronic acid / Hyaluronan levels in your samples. This colorimetric assay is optimised for quantitative analysis in-vivo, tissue-derived hyaluronic acid / Hyaluronan and includes full step-by-step instructions.
The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products
Please note, that you may have to pay country-specific taxes and duties.
Method/Platform
Lateral flow, sandwich or competetive
Range/Assay Sensivity
5 pg DNA, equivalent to agarose gel electrophoresis
Test Principle
HybriDetect 2T is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for simultaneous detection of two different analytes such as proteins, antibodies or gene amplification products. The results can be interpreted qualitative or quantitative.
Document
The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products
