NGS Library Quantification Kit (for Small RNA-Seq)
Facebook
X
Pinterest
Email
Detail
Overview
Able to quantify NGS Library (Illumina) of a wide spectrum of concentrations, including sub-nanomolar concentrations
DNA is accurately quantified by using a standard curve constructed from the provided DNA Standards
Specially designed DNA standards for Small RNA-Seq library; also compatible to NGS library of other molecular weights
Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations. The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.
Storage Conditions Upon receipt, store Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
For plate-based colorimetric enzymatic determination of alkaline phosphatase:
Distributed in almost every tissue of the body, serum alkaline phosphatase (ALP) levels are of interest in the testing for hepatobiliary disorder and bone disease. Most of the ALP in the normal adult serum is from the liver or biliary tract. Normal alkaline phosphatase levels are age-dependent and are elevated during periods of active bone growth. Moderate elevations of ALP (not involving the liver or bone) may be attributed to Hodgkin’s disease, congestive heart failure, and abdominal bacterial infections.
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma, and sarcoidosis.
The kit contains sufficient materials to rapidly test 42 samples in duplicate.
Document
Highly Sensitive, rapid, robust screening kit for sample phosphatase activity.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate
Applications
PCR, southern bolt and virus detection, etc
Purification method
96 well plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principle
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
High throughput – 96 samples can be processed simultaneously
Kit Contents
Contents
D311701
D311702
Purification Times
1 x 96
4 x 96
HiPure gDNA Plate
1
4
96 well Plate (2.2ml)
1
4
1.6ml Collection Plate
1
4
0.5ml Collection Plate
1
4
Silicon Seal Tape
1
4
Seal Film
5
25
Buffer ATL
30 ml
100 ml
Buffer AL
30 ml
100 ml
Buffer DW1
60 ml
250 ml
Buffer GW2
50 ml
2 x 100 ml
Proteinase K
50 ml
200 ml
Protease Dissolve Buffer
5 ml
15 ml
Buffer AE
30 ml
120 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
