Fast and easy recovery of DNA from agarose gel fragments
High recovery of desired DNA
Convenient spin column format
DNA is ready for ligation, restriction digestion, sequencing and more
This kit is designed for the rapid preparation and purification of DNA fragments that have been fractionated on agarose gels. The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing. The protocol can be completed in 20 minutes.
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component
Cat. 13100 (50 preps)
Binding Buffer G
80 mL
Wash Solution A
12 mL
Elution Buffer B
8 mL
Spin Columns
50
Collection Tubes
50
Elution Tubes (1.7 mL)
50
Product Insert
1
Other Products
D3129 HiPure Insect DNA Kit
Product Info
Document
Product Info
Introduction
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from <10 mg insect tissue
Applications
PCR, southern bolt and virus detection, etc
Purification method
Midi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Insect tissue samples
Sample amount
<10 mg
Elution volume
≥15μl
Time per run
≤30 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
Fast – several samples can be extracted in less than 30 minutes
Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
Safe – without phenol chloroform extraction and alcohol precipitation
Kit Contents
Contents
D312902
D312903
Purification Times
50
250
HiPure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Buffer ITL
30 ml
120 ml
Buffer IL*
30 ml
120 ml
Buffer GW1*
22 ml
110 ml
Buffer GW2*
20 ml
2 x 50 ml
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
15 ml
Buffer AE
15 ml
60 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
Document
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
[DL4000] ExcelDye™ 6X DNA Loading Dye, Tri-color, 5 ml x 2
Product Info
Document
Product Info
Description
The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.03% Xylene cyanol FF
0.03% Bromophenol blue
0.15% Orange G
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months -20°C for 36 months
Document
The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Cerillo’s Alto Plate Reader is a third party module that can be placed next to or inside the OT-2 or Opentrons Flex for post experimental analysis. The worktable adapter will allow you to utilize the OT-2 or Opentrons Flex to prepare the samples directly into the plate reader positions on the worktable.
This powerful optical system provides flexible calibration modes, accommodating a wide array of workflows including ELISA and microbial growth curves with diverse starting points. This plate reader is compatible with Cerillo’s Canopy wireless device that allows real time visualization, control, and analytics. This product is compatible with reading 450nm or 600nm wavelengths.
Document
Cerillo’s Alto Plate Reader is a third party module that can be placed next to or inside the OT-2 or Opentrons Flex for post experimental analysis. The worktable adapter will allow you to utilize the OT-2 or Opentrons Flex to prepare the samples directly into the plate reader positions on the worktable.
This powerful optical system provides flexible calibration modes, accommodating a wide array of workflows including ELISA and microbial growth curves with diverse starting points. This plate reader is compatible with Cerillo’s Canopy wireless device that allows real time visualization, control, and analytics. This product is compatible with reading 450nm or 600nm wavelengths.
