Overview
- High quality DNA, free from RNA contamination
- Isolate genomic DNA from anticoagulated and untreated blood
- Rapid and convenient spin column procedure
- Isolate DNA from inputs as low as 20 µL
This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column. The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes. The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.
Details
Supporting Data
Figure 1 / 2
Click for expanded view
| Kit Specifications | |
| Maximum Blood Input | 3 x 3 mm diameter punches |
| Average yield* (ng) | 150 ng |
| Column Binding Capacity | > 50 µg |
| Time to Complete 10 Purifications | 35 minutes |
* Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 36000 (50 preps) |
|---|---|
| Digestion Buffer B | 6 mL |
| Lysis Buffer B | 20 mL |
| Wash Solution WN | 18 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 1.2 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes | 50 |
| Product Insert | 1 |
