resDNASEQ CHO Residual DNA Quantitation kit (Without DNA Control )
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Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
Detail
Description
Features of there sDNASEQ CHO Residual DNA Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
Only one Reagent for qPCR;
Only 1.5 hours will be needed for the whole test.
Accurate
Perfect amplification curve, good amplification efficiency and good precision.
Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
Limit of Detection (LOD): 0.01 fg/μL; Limit of Quantification (LOQ): 0.3 fg/μL
The recovery rate of different concentration samples in the linear range is between 70% and 130%.
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1fg/μL, 0.3fg/μL, 0.5fg/μL, 1fg/μL and 3fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentration values of samples with 0.3fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
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Permagen’s XLE PCR-96 low volume bar magnet plate for PCR applications features an individual bar magnet for each row of wells, making it the perfect solution for hand pipetting as all of the pelleted beads will be to the same side in each row. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips
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Permagen’s XLE PCR-96 low volume bar magnet plate for PCR applications features an individual bar magnet for each row of wells, making it the perfect solution for hand pipetting as all of the pelleted beads will be to the same side in each row. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips
ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits
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Overview
Columns bind proteins of interest while endotoxins flow through
Reduce endotoxin levels to less than 0.01 EU/µg of protein
Proteins are desalted
Greater than 95% protein recovery
Concentrate protein samples and remove detergents at the same time
Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.
Mini Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Maxi Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Including Triton® X-100, CHAPS, NP-40 and Tween 20
Minimum Elution Volume
50 μL
Time to Complete 10 Purifications
20 minutes
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
Component
Cat. 22800 (25 preps)
Cat. 22200 (4 preps)
Binding Buffer J
8 mL
8 mL
Binding Buffer N
4 mL
20 mL
Wash Solution M
50 mL
130 mL
Wash Solution CIP
20 mL
60 mL
Wash Solution N
30 mL
130 mL
Wash Solution NIP
20 mL
60 mL
Elution Buffer G
6 mL
20 mL
Endotoxin Removal Solution
1.5 mL
1.5 mL
Protein Neutralizer EF
2 mL
2 mL
Maxi Spin Columns (assembled with Collection Tubes)
–
4
Mini Spin Columns
25
–
Collection Tubes
25
–
Maxi Spin Columns (assembled with Collection Tubes)
Norgen’s Nuclease-Free Water is free of all DNases, RNases and nucleic acids and is suitable for all molecular biology applications requiring nuclease-free water including PCR, RT-PCR and real-time PCR. Our water is prepared without the use of DEPC, and is ready to use without further treatment.