For scaling up and fully automating end-to-end NGS library prep
With the Flex NGS Workstation, you can automate your NGS workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. The Flex enables you to automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex NGS Workstation*
For scaling up and fully automating end-to-end NGS library prep
With the Flex NGS Workstation, you can automate your NGS workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. The Flex enables you to automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex NGS Workstation*
Our Blyscan™ Glycosaminoglycan Kit has been a ‘go-to’ Solution for reliable sGAG and Proteoglycan Analysis for many years! Blyscan utilises a dye-binding approach to quantitatively measure sulfated glycosaminoglycans (sGAG) and proteoglycans in cells, tissues and fluids from a wide range of in-vivo and in-vitro sources.
Colorimetric Detection (656nm) (Endpoint)
Glycosaminoglycans (GAGs) are a type of negatively charged polysaccharide that play crucial roles in various biological processes. They are composed of repeated disaccharide units, typically of N-acetylated or N-sulfated hexosamine paired with a uronic acid (GlcA or IdoA) or galactose. Sulfate groups can also be added to give sulfated GAGs an overall negative charge that influences cell interactions and also enable binding by our Blyscan dye reagent.
Common examples of GAGs include Chondroitin Sulfate, Dermatan Sulfate, Heparin, Heparan Sulfate, and Keratan Sulfate. Note that Hyaluronic Acid is a non-sulfated GAG and cannot be detected by the Blyscan assay. If you need to measure hyaluronic acid instead, we recommend using our Purple-Jelley kit!
GAGs and proteoglycans have essential functions in tissues and organisms, providing biophysical support through scaffolding and maintaining cartilage hydration. They also play a vital role in biochemical processes such as cell adhesion and signalling.
Blyscan is an Old English word meaning ‘to shine’ and from which the word ‘blush’, (blushing), may have been derived. This was an appropriate choice as the Blyscan Assay contains a blue dye which ‘blushes’ bright pink when it binds to sulphated glycosaminoglycans!
Step 1. Blyscan dye reagent contains DMMB dye in an optimised buffer. Addition of Dye reagent to samples containing sGAG results in the formation of a dye/sGAG complex due to a charge interaction between dye and GAG sulfate groups.
Step 2. Over a 30 minute incubation Dye-labelled sGAGs precipitate out of solution and are collected by centrifugation. Following removal of unbound dye, the remaining bound dye is released from the complex by
addition of dye dissociation reagent. Released dye is quantified spectrophotometrically.
Step 3. The sGAG content of unknown samples may be quantified by comparison against a calibration curve prepared using a standard of purified Chondroitin-4-sulfate supplied with the kit.
A list of suggested sample types can be found under the ‘Assay Specification‘ tab.
The Blyscan Dye reagent is formulated to miminise binding to other charged sample components such as nucleic acids, a problem with some older dye-based sGAG assays.
2.5 – 50µg/ml
2.5µg/ml
Colorimetric Detection (656nm) (Endpoint)
110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve).
In-vivo: Solid samples, including cartilage, bone, connective tissue, tumour tissue
In-vivo: Liquid samples, including fluids such as urine, amniotic or synovial fluid.
In-vitro: Solid samples, such as deposited ECM on 2D/3D culture surfaces.by enzymatic treatment
In-vivo: Liquid samples, Culture media during 2D/3D cell culture.
The assay requires that sulfated polysaccahrides or sGAGs are in a soluble form. A preliminary enzymatic extraction step is required for solid samples (enzyme not supplied with kit).
The assay is not suitable for use with samples containing alginates or that comprise degraded sulfated disaccharide fragments.
This kit is designed for research use only. Not for use in diagnostic procedures.
Kit requires access to a centrifuge, as well as a spectrophotometer/colorimeter capable of absorbance detection at 656nm.
Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.
1. Blyscan Dye Reagent (1x110ml)
2.sGAG Reference Standard (1x5ml, 100µg/ml Bovine tracheal chondroitin 4-sulfate)
3. Dissociation Reagent (1x110ml)
4. Sodium Nitrite (1x15ml)
5. Acetic Acid (1x15ml)
6. Ammonium Sulfamate (1x15ml)
7. 1.5ml micro-centrifuge tubes for dye-labelling reaction.
8. Assay kit manual
NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.
Our Blyscan™ Glycosaminoglycan Kit has been a ‘go-to’ Solution for reliable sGAG and Proteoglycan Analysis for many years! Blyscan utilises a dye-binding approach to quantitatively measure sulfated glycosaminoglycans (sGAG) and proteoglycans in cells, tissues and fluids from a wide range of in-vivo and in-vitro sources.
Colorimetric Detection (656nm) (Endpoin
Methyltetrazine-PEG23-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Methyltetrazine-PEG23-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Figure 1 / 4
Click for expanded view
| Kit Specifications | |
| Maximum Column Binding Capacity | 50 μg for RNA 20 μg for DNA 200 μg for protein |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Size of DNA Purified | ≥ 30 kb |
| Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues | 5 x 106 cells 25 mg (for selected tissues) 100 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg |
| Time to Complete 10 Purifications | 30 minutes |
| Average Yield:HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells) | 15 μg RNA8 μg DNA150 μg protein |
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 47700 (50 preps) | Cat. 51600 (50 preps) | Cat. 51700 (96 preps) |
|---|---|---|---|
| Buffer SKP | 40 mL | 40 mL | – |
| Lysis Buffer Q | – | – | 40 mL |
| Wash Solution A | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL |
| Elution Solution A | 6 mL | 6 mL | 20 mL |
| Elution Buffer F | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution C | 30 mL | 30 mL | 60 mL |
| Binding Buffer A | 8 mL | 8 mL | 8 mL |
| Elution Buffer C | 8 mL | 8 mL | 30 mL |
| Protein Neutralizer | 4 mL | 4 mL | 4 mL |
| Protein Loading Dye | 2 mL | 2 mL | 3 x 2 mL |
| gDNA Purification Columns | 50 | – | – |
| gDNA Purification Micro Columns | – | 50 | – |
| gDNA Purification 96-Well Plate | – | – | 1 |
| RNA/Protein Purification Columns | 50 | – | – |
| RNA/Protein Purification Micro Columns | – | 50 | – |
| RNA/Protein Purification 96-Well Plate | – | – | 1 |
| Collection Tubes | 150 | 150 | – |
| Collection Plate | – | – | 5 |
| Elution Tubes (1.7 mL) | 150 | 150 | – |
| Elution Plate | – | – | 3 |
| Lysis Preparation Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| Product Insert | 1 | 1 | 1 |
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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