For scaling up and fully automating end-to-end NGS library prep
With the Flex NGS Workstation, you can automate your NGS workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. The Flex enables you to automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex NGS Workstation*
For scaling up and fully automating end-to-end NGS library prep
With the Flex NGS Workstation, you can automate your NGS workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. The Flex enables you to automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex NGS Workstation*
E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.
E.coli O157:H7 TaqMan PCR Kit, 100 reactions
E.coli O157:H7 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM41350 (100 preps) | Cat. TM41310 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| E. coli O157:H7 Primer & Probe Mix | 280 μL | 280 μL |
| E. coli O157:H7 Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
| Usage | For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection. |
| Formula (per liter) | Peptone—————10.0g Beef Extract———–3.0g Sodium Chloride—–5.0g Final pH———–7.4 ± 0.2 |
| How to use | 1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121ºC for 15 minutes. 2.Diluted and treated samples. |
| Storage | Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years. |
500g
K-GCROLGK
SKU: 700004293
70 assays (manual) / 700 assays (microplate) / 600 assays (auto-analyser)
| Content: | 70 assays (manual) / 700 assays (microplate) / 600 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Glycerol |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 1.0 to 35 µg of glycerol per assay |
| Limit of Detection: | 0.37 mg/L |
| Reaction Time (min): | ~ 7 min |
| Application examples: | Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices, soft drinks, toothpaste, honey, tobacco, paper (and cardboard), cosmetics, pharmaceuticals, soap and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Novel method |
The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Explore our complete range of alcohol assay kit products.
Advantages
The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
