For scaling up and fully automating magnetic bead-based nucleic acid extraction
With the Flex Nucleic Acid Extraction Workstation, you can automate your NAE workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. Flex enables you to automate your nucleic acid isolation and purification workflows using any leading magnetic bead-based system on the market.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex Nucleic Acid Extraction Workstation*
Detail
For scaling up and fully automating magnetic bead-based nucleic acid extraction
With the Flex Nucleic Acid Extraction Workstation, you can automate your NAE workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. Flex enables you to automate your nucleic acid isolation and purification workflows using any leading magnetic bead-based system on the market.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex Nucleic Acid Extraction Workstation*
Other Products
Purple-Jelley Hyaluronic Acid assay kit
Product Info
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Product Info
Purple-Jelley Hyaluronic Acid assay kit
Biocolor’s Purple-Jelley assay kit is the perfect tool for accurate measurement of hyaluronic acid / Hyaluronan levels in your samples. This colorimetric assay is optimised for quantitative analysis in-vivo, tissue-derived hyaluronic acid / Hyaluronan and includes full step-by-step instructions.
Colorimetric Detection (655nm) (Endpoint)
Hyaluronic Acid: A gentle giant!
Hyaluronic acid, in its hydrated form, is a unique carbohydrate polymer, often referred to as a ‘gentle giant.’ It consists of a lengthy, flexible, non-branching chain with a repeating disaccharide pattern. This disaccharide is composed of alternating uronic acid and aminosugar units.
Why is our kit called ‘Purple-Jelley’?
Discovering the J-Aggregate Effect in Cyanine DyesIn 1936, Edwin Jelley made a fascinating observation, documented it in a letter to Nature (Nature 138, 1009 – 1010). He noted a peculiar behaviour of certain cyanine dyes, that when dissolved in 5 M NaCl, they dyes exhibited a third absorbance peak at a longer wavelength, around 650nm. In deionized water, however, they displayed only a double peak at approximately 540nm and 570nm. The 650nm peak in concentrated dye solutions resulted from the aggregation of dye molecules and was later termed a ‘J-aggregate,’ in honor of Edwin Jelley. The J-aggregate is known as a supra-molecular complex, formed by stacking individual dye molecules.
Subsequent research in the 1960s, notably by Kay et al. (J. Physical Chem. 68, 1896 – 1906), revealed that various biological polymers, including proteins, DNA, polar lipids, and glycosaminoglycans, could also induce this third absorbance peak. This phenomenon led to the development of the Purple-Jelley assay, named after the purple color of the dye reagent and Edwin Jelley himself.
An overview of the Purple-Jelley assay steps:
During the assay, hyaluronic acid is selectively purified during the assay sample preparation protocol. This is then reacted with the Purple-Jelley dye reagent, and the absorption of the characteristic third wavelength recorded. By comparison with a calibration curve the hyaluronic acid content of the sample can be measured.
Step 1. The assay protocol takes tissue samples through a sequential sample preparation protocol which involves enzymatic protein digestion, followed by precipitation and purification of GAGs, culminating in the precipitation of purified Hyaluronic acid.
Step2. The processed sample is then incubated for 10 minutes with the Purple-Jelley dye reagent, forming a coloured product which can be measured spectrophotometrically.
Step 3. The Hyaluronic acid content of unknown samples can be calculated by comparison against a calibration curve prepared using a standard comprising hyaluronic acid (supplied with the kit).
Assay range
10 – 100µg/ml
Limit of Detection
10µg/ml
Detection Method
Colorimetric Detection (655nm) (Endpoint)
Measurements per kit
100 in total (allows a maximum of 46 samples to be run in duplicate alongside a standard curve).
Suitable Samples
In-vivo: Hyaluronic acid purified from in-vivo tissues. The kit protocol involves extraction and purification of hyaluronic acid prior to reaction with the Purple-Dye reagent.
Precautions
This kit is designed for research use only. Not for use in diagnostic procedures. Kit requires access to a centrifuge, as well as a spectrophotometer/colorimeter capable of colorimetric, absorbance detection at 655nm. Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.
2. Hyaluronan Reference Standard (1x 5ml, 0.2mg/ml soluble Hyaluronic Acid)
3. Precipitating Reagent (2x 34ml)
4. Sodium Chloride (1x 20ml)
5. Cetylpyridinium Chloride (1x 20ml)
6. TRIS-buffered Saline (5x tablets)
7. 2ml screw-cap tubes for preparation of samples.
8. Assay kit manual
NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.
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Biocolor’s Purple-Jelley assay kit is the perfect tool for accurate measurement of hyaluronic acid / Hyaluronan levels in your samples. This colorimetric assay is optimised for quantitative analysis in-vivo, tissue-derived hyaluronic acid / Hyaluronan and includes full step-by-step instructions.
A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.
Details
Specifications
Features
Specifications
Main Functions
Removal of free fluorescent dye from sequencingsolution (Replace Beckmen or agencourt CleanSeq)
Applications
Automated sequences
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
DNA doped with fluorescentdye
Sample amount
5μl
Recovery
90%
Elution volume
≥25μl
Operation time
≤50 minutes
Principle
The CleanSeq method contains magnetic particles in an optimized binding buffer to selectively capture sequencing extension products. The protocol can be performed directly in the thermal cycling plate. Unincorporated dyes, nucleotides, salts and contaminants are removed using a simple washing procedure. The purification procedure is amenable to a variety of automation platforms since it requires no centrifugation or vacuum filtration.
Advantages
High recovery – up to 90%
High throughput – using magnetic beads purification technology
CleanSeq Beads should be stored at 2-8°C upon arrival and is stable up to 6 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Mix CleanSeq Beads well before using. The reagent should appear homogenous and consistent in color.
DO NOT FREEZE.
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A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.
• For sizing and quantification of double strand DNA fragments. • Composed of 5 bands from 10 kb to 48.5 kb. • Premixed with 6X DNA loading buffer for direct gel loading.
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• For sizing and quantification of double strand DNA fragments.
• Composed of 5 bands from 10 kb to 48.5 kb.
• Premixed with 6X DNA loading buffer for direct gel loading.
