Opentrons Flex 1-Channel Pipette

Overview

• Detection kits for RSV-A
• Available in TaqMan format for analysis

Norgen’s Respiratory Syncytial Virus A (RSV-A) TaqMan RT-PCR Detection Kit is designed for the detection of RSV-A specific RNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of RSV-A specific RNA is based on TaqMan one-step RT-PCR providing a simple, reliable and rapid result for the detection of RSV-A infection. Norgen’s TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The RSV-A TaqMan RT-PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

RSV-A TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

RSV-A TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM34250 (100 preps)	Cat. TM34210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
RSV-A Primer & Probe Mix	280 μL	280 μL
RSV-A Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

K-PHYTASE

SKU: 700004328

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Phytase
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	360
Signal Response:	Increase
Linear Range:	0.1 to 10 μg of phosphate per assay
Limit of Detection:	1.5 U/L
Reproducibility (%):	< 7%
Reaction Time (min):	~ 30 min
Application examples:	Animal feeds, phytase activity in cereal, fungal and bacterial phytases.
Method recognition:	Novel method

The Phytase Assay Kit is a simple, quantitative method which can be used to measure phytase activity. Analysis is based on the hydrolysis of phytic acid by phytase and quantitative measurement of the phosphate released. Results are measured using a standard UV-VIS spectrophotometer and do not require the creation of a standard curve. The Phytase method can be used to measure phytase activity in cereal, fungal and bacterial phytases, and can also be used for the analysis of phytase in animal feed samples.

View our complete list of assay kits for enzyme activities.

Advantages

• Easier and quicker test than competitors
• No need for expensive instrumentation, i.e. fluorimeter
• Low cost per assay
• All reagents stable for > 2 years after preparation
• Standard included
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing