The Opentrons Flex Waste Chute GEN1 occupies position D3 and is used to accept waste such as tips, tip racks, and plates in high volumes. The gripper will pick up the consumable and dispose it into the chute based on your workflow needs. The waste chute is neccesary for all high-throughput workflows and can be paired with the Opentrons Flex Deck Expansion Set to maximize the throughput capabilities of the Opentrons Flex. The following consumables can disposed of into the waste chute:
– NEST 2 mL Deep Well Plate, V Bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 well Plate Flat
– All Opentrons Flex tip racks
Detail
The Opentrons Flex Waste Chute GEN1 occupies position D3 and is used to accept waste such as tips, tip racks, and plates in high volumes. The gripper will pick up the consumable and dispose it into the chute based on your workflow needs. The waste chute is neccesary for all high-throughput workflows and can be paired with the Opentrons Flex Deck Expansion Set to maximize the throughput capabilities of the Opentrons Flex. The following consumables can disposed of into the waste chute:
– NEST 2 mL Deep Well Plate, V Bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Document
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
For the rapid detection and enumeration of Escherichia coli.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine coli β- glucuronidase enzyme specific reaction, hydrolysis of the substrate, the release of the color groups produce green colonies on the light yellow plate.
Formulation (per liter):
Peptone :15.0g
Yeast extract powder: 3.0g
Sodium chloride: 5.0g
Sodium lauryl sulfate:0.1g
Agar: 12.0g
Chromogenic substrate 6.5g
Final pH 7.0 ± 0.2
How to use:
1. Weigh 41.6g of the product, adding 1.0L distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, 115 autoclaved for 10minutes.
2. Take 25.0g or 25.0ml of sample with sterile procedures , added to the flask containing 225.0mL of sterile phosphate buffered saline (or saline) , shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 medium.
3, observe the results.
Quality Control
This product appears light yellow after the pouring on plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.
Bacteria name Bacteria NO. Growth Situation Feature
Escherichia coli ATCC25922 good green colonies
Citrobacter ATCC8090 good colorless colonies
Salmonella typhimurium CMCC50115 good colorless colonies
Enterococcus faecalis ATCC29212 suppressed —–
Storage: Store in a dark, cool and dry place, tighten the caps immediately after use. Storage period of two years.
