Aluminum Block Set

Overview

• Fast and easy processing using a rapid spin-column format
• Isolate high quality genomic DNA from fresh or preserved saliva samples
• Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000)

This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000), as well as fresh saliva samples.

Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Background

Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.

Details

Supporting Data

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Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	3 – 7 μg
Average Purity (OD260/280)	1.7 – 2.1
Time to Complete 10 Purifications	30 minutes

* Average yield will depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. RU45400 (50 preps)
Lysis Buffer F	30 mL
Proteinase K in Storage Buffer	1.2 mL
Binding Buffer B	12 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Description

Specifications

Clone	IHC531
Source	Mouse Monoclonal
Positive Control	Tonsil
Dilution Range	1:200

Cluster of differentiation 2 (CD2) is a useful early T-cell lineage restricted antigen that is present in T-cell differentiation. As a pan-T-cell marker, CD2 staining is used for recognizing practically all normal T-cells, but may be deleted in some T-cell neoplasms. Since CD2 is present in most precursor and mature T-cell leukemias and lymphomas, it is useful in the evaluation of lymphoid malignancies. By using CD2 and CD25 staining, the recognition of systemic mastocytosis and mastocytic leukemia is supported.

Heat Sealing Time:		0.5~2s			Heat Sealing Tube Outer Diameter:		2~6mm
Indicator Light Colors:		Red/Blue			RF Frequency:		40.68MHz
Grounding Protection:		Class l			Spacing Adjustment		65,70,75,80,85,90,95,100mm
Power Consumption:		250W during operation,10Win standby			Power Supply:		100V~120V/220V~240V AC
Weight:		4.5kg			Dimensions:		336×65×173mm
Operating Temperature:		0~40℃			Storage Temperature:		-20~70℃

A device that uses the principle of high-frequency oscillation to generate heat through the friction of molecules in PVC hoses, thereby melting the PVC hose and achieving the sealing effect. This device is also referred to as a pipe sealing machine in some places.