Aluminum Block – 96-well PCR Plate

Overview

• Purification and enrichment of intact cell culture media exosomes for functional studies
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• Versatile cell culture media input volumes
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents nor overnight incubation required
• Pure exosomes are purified and are free from any other RNA-binding proteins
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

For sample input volumes ranging from 5 mL to 10 mL.

Cell Culture Media Exosome Purification and RNA Isolation Midi Kit

For sample input volumes ranging from 10 mL to 20 mL.

Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit

For sample input volumes ranging from 20 mL to 35 mL.

Details

Supporting Data

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Kit Specifications
Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	20 mL to 35 mL
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40 to 45 minutes
Average Yields*	Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component	Cat. 60700 (50 preps)	Cat. 60800 (25 preps)	Cat. 60900 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	1.5 mL	1.5 mL	1.5 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	20 mL	20 mL	20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	18 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Mini Filter Spin Column	50	25	15
Mini Spin Columns	50	25	15
Collection Tubes	50	25	15
Elution tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) consists of two terminal propargyl groups and an aminooxy group. The propargyl groups reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The aminooxy group is reactive towards aldehydes to form oxime bonds. If a reductant is used, it will form a hydroxylamine linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.

N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) consists of two terminal propargyl groups and an aminooxy group. The propargyl groups reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The aminooxy group is reactive towards aldehydes to form oxime bonds. If a reductant is used, it will form a hydroxylamine linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.

Description

Spring Viremia of Carp is caused by Rhabdovirus carpio, a bullet-shaped RNA virus. The disease has been reported in common carp (or koi) (Cyprinus carpio), grass carp (Ctenopharyngodon idella), bighead carp (Aristichthys nobilis), silver carp (Hypophthalmichthys molitrix), and Crucian carp (Carassius carassius), a close relative of the goldfish. Recent evidence suggests that common goldfish (C. auratus) are also susceptible.
The disease was initially diagnosed in Yugoslavia (Fijan et al. 1971). Since then, it has been identified in other European countries, Russia, and the Middle East. Mortality has reached 70% in yearling carp from European populations. Adult fish can also be affected but to a lesser degree.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings