Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.
Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Rapidly Extract viral DNA/RNA from 200μl non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, etc. |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral DNA / RNA, body cell DNA / RNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Plasma, serum, effusion, urine, fecal suspension supernatant |
| Sample amount | 200μl |
| Elution volume | ≥30μl |
| Time per run | |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
Kit Contents
| Contents | IVD4175 |
| Purification Times | 100 Preps |
| HiPure Viral Column | 100 |
| 2ml Collection Tubes | 100 |
| PK/Carrier RNA | 50 mg/310μg |
| Protease Dissolve Buffer | 5 ml |
| Buffer VLE | 42 ml |
| Buffer CE | 60 ml |
| RNase Free Water | 15 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
| Clone | IHC132 |
| Source | Mouse Monoclonal |
| Positive Control | Astrocytoma |
| Dilution Range | 1:200 |
Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytosolic enzyme involved in the TCA metabolic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumours, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma.
NEST 1.5 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
NEST 1.5 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
