Overview

• Detection kits for the HPV 6/16
• Available in TaqMan format for analysis

More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. HPV 16 has been considered as a high-risk cancer associated HPV type. The low-risk HPV type 6 has been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA.

HPV 6/16 TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

HPV 6/16 TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	TaqMan Probe Cat. TM42050 (100 preps)	TaqMan Probe/Primer and Control Set Cat. TM42010 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 µL	–
HPV 6/16 Primer & Probe Mix	280 µL	280 µL
HPV 6/16 Positive Control	150 µL	150 µL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Bacterial DNA Extraction Kit (Magnetic Beads)

The Bacterial DNA Extraction Kit (Magnetic Beads) was developed for the bacterial genomic DNA extraction from bacterial cultures directly using magnetic beads from a wide variety of gram-negative and gram-positive bacterial species, as well as yeast and other fungi. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality bacterial DNA isolation with fast magnetic response time and high binding capacity.

Bacteria are the most diverse and abundant small single-celled organisms and are vital to the planet’s ecosystems. Some bacterial species are pathogens that can cause a variety of diseases to humans and animals. Besides their ecological and biomedical importance, bacteria are also used in biotech and pharmaceutical applications such as production of enzymes, DNA preparation, biofuels, food research, and chemical production.

Bacterial cells are grown to log-phase and the culture is lysed directly with a lysis buffer, then mixed with beads to bind genomic DNA. After wash steps, genomic DNA is eluted in Low TE or TE Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities. Bacterial DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, Southern Blotting, molecular cloning, DNA hybridization, restriction enzymatic digestion, and Next-generation Sequencing (NGS) etc.

Features

• 100% centrifuge-free
  • Bacterial cultures can be used directly without centrifuge to pellet the bacteria
• Simple magnetic beads method
  • No centrifuge needed
  • No column needed
  • No vacuum needed

Purified genomic DNA from various bacteria were isolated with the Bacterial DNA Extraction Kit. A portion of the extracted genomic DNA was loaded on a 1% agarose gel. DNA ladder: BioDynami 1 kb Plus DNA Ladder (Cat.# 10005L).