NEST 1.5 mL Microcentrifuge Tube (500 count)

TDL5B refrigerated low speed centrifuge

Technical Parameters

Matched Rotors for TDL5B

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 50 & 150 reactions

ssDNA Quantification Kit

The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.

Our kit detects ssDNA by using fluorescent dye that enables sensitive single stranded DNA quantification , including ssDNA viruses, synthetic ssDNA, first-strand cDNA synthesis, denatured DNA, and bisulfate-converted DNA etc. ssDNA quantification is essential for the study of the biological process involves ssDNA.

Features

• • • Optimized for use with the Qubit® Fluorometer
    • Uses the Qubit® ssDNA assay setting
    • Linear range: 1-200 ng ssDNA
    • Cost saving by more than 50%

A series of input ssDNA (200, 400, 600, 800, 1000, and 1200 ng) was used.

The performance of the BioDynami ssDNA Quantification Kit is nearly identical to that of Thermo Fisher’s Qubit ssDNA kit (figure below).

Comparison of BioDynami ssDNA Quantification Kit with Thermo Fisher kit.

Common contaminants such as salts, solvents, or detergents are well tolerated in the assay (Table 1).

Contaminants has been tested in BioDynami ssDNA Kit.

The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.