NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free
Conforms to SBS and ANSI requirements
Centrifugal strength up to 3000g
Square well
50 count
Labware definition is available for immediate use in Opentron’s Labware Library
NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free
Conforms to SBS and ANSI requirements
Centrifugal strength up to 3000g
Square well
50 count
Labware definition is available for immediate use in Opentron’s Labware Library
Non-woven gas permeable seal that limits evaporation. The seal is peelable, pierceable; suitable for cell culture and seed & insect storage too.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from bacterial culture |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Gram negative or positive bacteria |
| Sample amount | Bacterial culture: 0.5-1.5ml |
| Elution volume | ≥60μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D513101 | D513102 | D513103 |
| Purification Times | 48 Preps | 96 Preps | 480 Preps |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml | 30 ml |
| Buffer SDS | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Buffer GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Storage and Stability
MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Name of Product
IL-6 / Interleukin-6 Test
Catalog Number
MQL6 1
Short Info
Test for the semi-quantitative evaluation of human Interleukin-6 (IL-6) in serum, plasma, cell culture supernatant, amnitotic fluid or cerebrospinal fluid.
This product is only available within the EU!
Method/Platform
lateral flow
Range/Assay Sensivity
50 to 10 000 pg/ml
Test Principle
IL-6 binds to a first anti IL-6 antibody conjugated to gold particles. The IL-6 loaded gold particles diffuse through the membrane and overflow the test line.
A second monoclonal antibody, specific for IL-6 is coated on the membrane. The gold particles are bound specifically and become visible as a coloured line. Colour intensity is directly proportional to the concentration of IL-6 in the sample. The conjugated specific antibodies printed as a second line on the membrane captures the rest of gold conjugate.
20 tests
Test for the semi-quantitative evaluation of human Interleukin-6 (IL-6) in serum, plasma, cell culture supernatant, amnitotic fluid or cerebrospinal fluid.
